[{"@context":"http:\/\/schema.org\/","@type":"BlogPosting","@id":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/16999#BlogPosting","mainEntityOfPage":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/16999","headline":"\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA-\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc – \u30a6\u30a3\u30ad\u30da\u30c7\u30a3\u30a2","name":"\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA-\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc – \u30a6\u30a3\u30ad\u30da\u30c7\u30a3\u30a2","description":"before-content-x4 taq – \u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3092\u5099\u3048\u305fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08\u5de6\u4e0a\uff09\u304a\u3088\u3073DNA\u3092\u4f34\u3046\u30dd\u30ea\u30de\u30fc\u578b\uff08\u53f3\u4e0b\uff09 \u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA-\u30dd\u30ea\u30e1\u30e9\u30bb\u30f3 \u71b1\u7dda\u7cfb\u304b\u3089\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3042\u308a\u3001\u307b\u3068\u3093\u3069\u304c\u7d30\u83cc\u307e\u305f\u306f\u53e4\u7d30\u83cc\u306e\u7a2e\u3067\u3042\u308a\u3001\u3057\u305f\u304c\u3063\u3066\u71b1\u5b89\u5b9a\u6027\u3067\u3059\u3002\u3053\u308c\u3089\u306f\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\u3068\u3001DNA\u306e\u91cd\u8907\u3068\u4fee\u98fe\u306e\u305f\u3081\u306e\u95a2\u9023\u65b9\u6cd5\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 after-content-x4 \u5929\u7136\u8d77\u6e90\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u7279\u306b\u71b1\u6027\u7d30\u83cc\u3001\u89d2\u304a\u3088\u3073\u305d\u306e\u75c5\u539f\u6027\u3067\u767a\u751f\u3057\u307e\u3059\u3002\u7d30\u83cc\u306e\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4e0b\u3067\u306f\u3001Taq\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 TFL – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bcthat TMA – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bcthat tne – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3068 tth – \u4f7f\u7528\u3057\u305f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3002","datePublished":"2022-08-10","dateModified":"2022-08-10","author":{"@type":"Person","@id":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/author\/lordneo#Person","name":"lordneo","url":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/author\/lordneo","image":{"@type":"ImageObject","@id":"https:\/\/secure.gravatar.com\/avatar\/44a4cee54c4c053e967fe3e7d054edd4?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/44a4cee54c4c053e967fe3e7d054edd4?s=96&d=mm&r=g","height":96,"width":96}},"publisher":{"@type":"Organization","name":"Enzyklop\u00e4die","logo":{"@type":"ImageObject","@id":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/08\/download.jpg","url":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/08\/download.jpg","width":600,"height":60}},"image":{"@type":"ImageObject","@id":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/e\/ed\/Taq.png\/220px-Taq.png","url":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/e\/ed\/Taq.png\/220px-Taq.png","height":"188","width":"220"},"url":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/16999","wordCount":3237,"articleBody":"     (adsbygoogle = window.adsbygoogle || []).push({});before-content-x4 taq  – \u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3092\u5099\u3048\u305fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08\u5de6\u4e0a\uff09\u304a\u3088\u3073DNA\u3092\u4f34\u3046\u30dd\u30ea\u30de\u30fc\u578b\uff08\u53f3\u4e0b\uff09 \u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA-\u30dd\u30ea\u30e1\u30e9\u30bb\u30f3 \u71b1\u7dda\u7cfb\u304b\u3089\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3042\u308a\u3001\u307b\u3068\u3093\u3069\u304c\u7d30\u83cc\u307e\u305f\u306f\u53e4\u7d30\u83cc\u306e\u7a2e\u3067\u3042\u308a\u3001\u3057\u305f\u304c\u3063\u3066\u71b1\u5b89\u5b9a\u6027\u3067\u3059\u3002\u3053\u308c\u3089\u306f\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\u3068\u3001DNA\u306e\u91cd\u8907\u3068\u4fee\u98fe\u306e\u305f\u3081\u306e\u95a2\u9023\u65b9\u6cd5\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002        (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4\u5929\u7136\u8d77\u6e90\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u7279\u306b\u71b1\u6027\u7d30\u83cc\u3001\u89d2\u304a\u3088\u3073\u305d\u306e\u75c5\u539f\u6027\u3067\u767a\u751f\u3057\u307e\u3059\u3002\u7d30\u83cc\u306e\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4e0b\u3067\u306f\u3001Taq\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 TFL  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bcthat TMA  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bcthat tne  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3068 tth  – \u4f7f\u7528\u3057\u305f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3002 [\u521d\u3081] [2] [3] A\u578bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u5c5e\u3059\u308b\u7d30\u83cc\u306e\u71b1\u30b9\u30bf\u30d3\u30ea\u30c3\u30afDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u30015 ‘\u21923’-\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u6d3b\u6027\u3092\u6301\u3063\u3066\u3044\u307e\u3059 5 ‘\u21923’-\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027 \u30a2\u30c7\u30ce\u30b7\u30f3\u30aa\u30fc\u30d0\u30fc\u30cf\u30f3\u30b0\uff08\u82f1\u8a9e\uff09\u3092\u4f5c\u6210\u3057\u307e\u3059\u3002 \u82f1\u8a9e  \u7c98\u7740\u6027\u306e\u7aef \uff09\u3002\u30d7\u30ed\u30bb\u30b9\u6027\uff08\u82f1\u8a9e \u52a0\u5de5\u6027 \uff09DNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u524d\u306e\u5869\u57fa\u5bfe\u306e\u5e73\u5747\u6570\u3092\u8aac\u660e\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u30ec\u30f3\u30d7\u30ec\u30fc\u30c8 \uff09\u30c9\u30ed\u30c3\u30d7\u3002\u4f7f\u7528\u3055\u308c\u305f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u52a0\u5de5\u6027\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306e\u5b9a\u91cf\u7684PCR\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6700\u5927\u8ddd\u96e2\u3092\u30d7\u30ed\u30fc\u30d6\u306b\u5236\u9650\u3057\u307e\u3059\u3002 TAQ\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u52a0\u5de5\u6027\u306f\u3001\u7d04200\u5869\u57fa\u5bfe\u3067\u3059\u3002        (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4 PFU  –  2\u3064\u306e\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\uff08\u7070\u8272\u306e\u30dc\u30fc\u30eb\uff09\u3092\u5099\u3048\u305f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc B\u30bf\u30a4\u30d7\u306e\u983b\u7e41\u306b\u4f7f\u7528\u3055\u308c\u308bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u53e4\u7d30\u83cc\u306ePFU\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u3059\u3002 [\u521d\u3081] PWO\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 \u30b3\u30fc\u30c9  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [4]  tli -polymerase\uff08\u307e\u305f \u6392\u51fa\u3059\u308b \u547c\u3070\u308c\u305f\uff09\u3001 [5]  \u9b3c\u3054\u3063\u3053  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [6]  tce  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [7]  tgo  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [8]  TNA1  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [9]  TPE  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [\u5341]  tthi  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001 [11]  neq  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc [12\u756a\u76ee] \u305d\u3057\u3066\u305d\u306e \u30d8\u30eb\u30d7  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3002 [13] B\u578b\u306b\u5c5e\u3059\u308b\u53e4\u98a8\u306a\u30d0\u30ea\u30a2\u30f3\u30c8\u306f\u3001\u30aa\u30fc\u30d0\u30fc\u30cf\u30f3\u30b0\u3092\u4f5c\u6210\u3057\u307e\u305b\u3093\u3002 \u920d\u3044\u7d42\u308f\u308a \u3001 tli -polymerase\u306f\u88fd\u54c1\u306e\u7d0430\uff05\u3067\u5f35\u308a\u51fa\u3057\u307e\u3059\uff09 5 ‘\u21923’-\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027 \u5408\u6210\u30a8\u30e9\u30fc\u3092\u4fee\u6b63\u3059\u308b\u305f\u3081\u306e\u30a2\u30af\u30c6\u30a3\u30d3\u30c6\u30a3\u3002 \u6821\u6b63 \uff09\u3001 3 ‘\u21925’-\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027 \u3002 [14] [15] \u53e4\u98a8\u306a\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3067\u306f\u3001\u77ef\u6b63\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u304c\u9664\u53bb\u3055\u308c\u308b\u305f\u3081\u3001\u985e\u4f3c\u306e\u30af\u30ec\u30ce\u30a6\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3092\u4f5c\u6210\u3059\u308b\u3068\u30a8\u30e9\u30fc\u7387\u304c\u4f4e\u4e0b\u3057\u307e\u3059\u3002 [\u521d\u3081] Archaeen\u306e\u4e00\u90e8\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u6a19\u6e96\u306ePCR\u306b\u5bfe\u3059\u308b\u9069\u5408\u6027\u306b\u3088\u3063\u3066\u3042\u307e\u308a\u7279\u5fb4\u4ed8\u3051\u3089\u308c\u3066\u3044\u307e\u305b\u3093\u304c\u3001AdNA\u306e\u5897\u5e45\u306b\u304a\u3051\u308b\u963b\u5bb3\u306e\u6e1b\u5c11\u306b\u3088\u3063\u3066\u306f\u3042\u307e\u308a\u7279\u5fb4\u4ed8\u3051\u3089\u308c\u3066\u3044\u307e\u305b\u3093\u3002 [16] \u3055\u307e\u3056\u307e\u306a\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30eb\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3068\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30ebDNA\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306eDNA\u30af\u30ea\u30c3\u30d7\u3067\u4f5c\u3089\u308c\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u8a2d\u8a08 SSO7D \u4f4e\u8aa4\u5dee\u7387\u306e\u53e4\u98a8\u306a\u8089\u3068\u751f\u6210\u3055\u308c\u305f\u7d30\u83cc\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u9ad8\u3044\u5408\u6210\u3092\u4f34\u3046\u7570\u306a\u308b\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08 Q5 -polymerase\uff09\u3002 [17] PCNA\u30db\u30e2\u30ed\u30b0\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea Archaeoglobus\u306f\u8f1d\u3044\u3066\u3044\u307e\u3059 \u53e4\u98a8\u306a\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u751f\u6210\u3055\u308c\u307e\u3059\u3002 [18] \u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u306e\u71b1\u5b89\u5b9a\u6027DNA\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u30c9\u30e1\u30a4\u30f3\u3092\u4f7f\u7528\u3057\u305f\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u985e\u4f3c\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u578bV\u306e\u578b\u3001 Helix-Hairpin-Helix-Motiv \u3001 HHH \uff09out \u30e1\u30bf\u30ce\u30d4\u30e9\u30b9\u30fb\u30ab\u30f3\u30c9\u30ec\u30ea \u751f\u6210\u3055\u308c\u305f\uff08 TOPOTAQ \u3068 PFUC2 \uff09\u3002 [19] [20] \u4fee\u6b63\u3055\u308c\u305f\u3082\u306e\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u8a2d\u8a08\u306b\u3088\u3063\u3066\u4f5c\u6210\u3055\u308c\u307e\u3057\u305f PFU  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u751f\u6210\uff08 PFU\u30a6\u30eb\u30c8\u30e9 \uff09\u3002 [21] \u540c\u69d8\u306e\u52b9\u679c\u306f\u3001\u4e21\u65b9\u306e\u30bf\u30a4\u30d7\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u6df7\u5408\u7269\u3067\u3082\u9054\u6210\u3055\u308c\u307e\u3059\u300230\u301c1\u306e\u30bf\u30a4\u30d7A\u304a\u3088\u3073B\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u9175\u7d20\u6d3b\u6027\u306e\u6df7\u5408\u6bd4\u3001 [\u5341] [22] z\u3002 B.\u6b7b\u306c \u30d8\u30eb\u30ab\u30e9\u30fc\u30bc \u306e\u5546\u696d\u7684\u306a\u30df\u30c3\u30af\u30b9\u3068\u3057\u3066 taq – \u305d\u3057\u3066\u305d\u306e PFU  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3002 [8]        (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4\u3055\u307e\u3056\u307e\u306a\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u57fa\u672c\u7684\u306a\u5100\u5f0f\uff08\u82f1\u8a9e \u751f\u7523\u6027 \uff09\u6bd4\u8f03\u3055\u308c\u307e\u3057\u305f\u3002 [8] \u306e\u5408\u6210 taq -polymerase\u306f\u3001\u7d0460\u5869\u57fa\u30da\u30a2\u3042\u305f\u308a\u3067\u3059\u3002\u5909\u66f4\u3055\u308c\u3066\u3044\u306a\u3044\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4e0b\u3067\u3001\u306e\u5408\u6210\u901f\u5ea6\u306e\u307f \u30b3\u30fc\u30c9  –  100\u79d2\u3042\u305f\u308a100\u30da\u30a2\u3092\u8d85\u3048\u308b\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08\u7d04120 bp\/s\uff09\u3002 [23] \u5408\u6210\u9178\u5869\u3092\u5897\u52a0\u3055\u305b\u308b\u4fee\u98fe\u3055\u308c\u305f\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u4e2d\u3067\u3055\u307e\u3056\u307e\u306a\u5909\u7570\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059\u3002 [24] [25]  \u30b3\u30fc\u30c9  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3068\u3044\u304f\u3064\u304b\u306e\u4fee\u98fe\u3055\u308c\u305f\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\uff08 iProof \u3001 PFU\u30a6\u30eb\u30c8\u30e9 \u3001 \u30d5\u30e5\u30fc\u30b8\u30e7\u30f3 \u3001 \u901f\u5ea6 \u307e\u305f z-taq \uff09\u5408\u6210\u7387\u304c\u9ad8\u3044\u305f\u3081\u3001\u77ed\u3044\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u3092\u5099\u3048\u305fPCR\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\uff08 \u9ad8\u901f-PCR \u3001 \u9ad8\u901fPCR \uff09\u3002 \u7570\u306a\u308b\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u30a8\u30e9\u30fc\u7387\uff08\u82f1\u8a9e \u5fe0\u5b9f \uff09\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u8aac\u660e\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u306e\u30a8\u30e9\u30fc\u7387 taq  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f8\u30fb10\u3067\u3059 -6 \u30d9\u30fc\u30b9\u30da\u30a2\u3054\u3068\u306e\u30a8\u30e9\u30fc \u30b3\u30fc\u30c9  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc3,5\u30fb10 -6 \u30d9\u30fc\u30b9\u30da\u30a2\u3054\u3068\u306e\u30a8\u30e9\u30fc tli  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3068 \u30d8\u30eb\u30ab\u30e9\u30fc\u30bc 2.8\u30fb10 -6 \u30d9\u30fc\u30b9\u30da\u30a2\u3054\u3068\u306e\u30a8\u30e9\u30fc PFU  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc1,3\u30fb10 -6 \u30d9\u30fc\u30b9\u30da\u30a2\u3054\u3068\u306e\u30a8\u30e9\u30fc\u3068\u306e\u30a8\u30e9\u30fc PFU\u30a6\u30eb\u30c8\u30e9 4.3\u30fb10 -7 \u30d9\u30fc\u30b9\u30da\u30a2\u3054\u3068\u306e\u30a8\u30e9\u30fc\u3002 [\u521d\u3081] [8] \u7d30\u83cc\u306e\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5834\u5408\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u985e\u4f3c\u3057\u3066\u3044\u307e\u3059 \u5927\u8178\u83cc \u30bf\u30f3\u30d1\u30af\u8cea\u8a2d\u8a08\u306e\u904e\u7a0b\u3067\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u30c9\u30e1\u30a4\u30f3\u306e\u524a\u9664\u3001\u30af\u30ec\u30ce\u30a6\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\uff08 \u30b1\u30eb – \u305d\u308c \uff09\u307e\u305f\u306f\u30d5\u30a1\u30d6\u30ea\u30c3\u30af\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u305d\u306e\u7d50\u679c\u3001\u88fd\u54c1\u6fc3\u5ea6\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002 [26] [2] \u306e\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u95a2\u6570\u306e2\u3064 taq  – \u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u5fc5\u8981\u306a\u30a2\u30df\u30ce\u9178\u306f\u3001\u7a81\u7136\u5909\u7570\u8a98\u767a\u306b\u3088\u3063\u306625\u304a\u3088\u307374\u306e\u4f4d\u7f6e\u3067\u30a2\u30eb\u30ae\u30cb\u30f3\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u307e\u3057\u305f\uff08R25\u304a\u3088\u3073R74\uff09\u3002 [27] \u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306b\u3088\u308b\u500b\u3005\u306e\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u597d\u307f\u306f\u3001\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u7279\u7570\u6027\u3067\u3059\uff08\u82f1\u8a9e\u3002 \u30d0\u30a4\u30a2\u30b9 \u300c\u597d\u307f\u300d\u3001\u300c\u30d0\u30a4\u30a2\u30b9\u300d\uff09\u3002\u30c1\u30a7\u30fc\u30f3\u7d42\u7aef\u57fa\u8cea\uff08Office Oxymethode\uff09\u3092\u4f7f\u7528\u3057\u305fPCR\u30d9\u30fc\u30b9\u306eDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3088\u308a\u3001\u305d\u308c\u3089\u306e\u5747\u4e00\u306a\u8a2d\u7f6e\u3001\u3057\u305f\u304c\u3063\u3066\u3001\u3059\u3079\u3066\u306e\u30c1\u30a7\u30fc\u30f3\u30d6\u30ec\u30a4\u30af\u30a2\u30a6\u30c8\u88fd\u54c1\u306e\u5747\u7b49\u306a\u4e16\u4ee3\u304c\u671b\u307e\u3057\u3044\u305f\u3081\u3001\u3088\u308a\u591a\u304f\u306e\u611f\u5ea6\u3068\u5bb9\u6613\u306a\u8a55\u4fa1\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002\u3053\u308c\u3092\u884c\u3046\u306b\u306f\u30011\u3064 \u30af\u30ec\u30f3\u30bf\u30c3\u30af  – \u524a\u9664\u306b\u3088\u3063\u3066\u751f\u6210\u3055\u308c\u3001\u5c40\u6240\u7279\u7570\u7684\u7a81\u7136\u5909\u7570\u8a98\u767a\uff08\u77ed\u3044\uff1aF667y\uff09\u3092\u4ecb\u3057\u3066\u30c1\u30ed\u30b7\u30f3\u306e\u4f4d\u7f6e667\u306e\u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30cb\u30f3\u3092\u4ea4\u63db\u3057\u305f\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc \u30b5\u30fc\u30e2\u30b7\u30fc\u30b1\u30ca\u30fc\u30bc \u5c02\u7528\u3002 [28] [29] \u3053\u306e\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u86cd\u5149\u30de\u30fc\u30af\u3055\u308c\u305f\u30c7\u30a3\u30b9\u30aa\u30ad\u30b7\u30ce\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u8a2d\u7f6e\u306b\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002 [30] andere dna-polymerasen [ \u7de8\u96c6 | \u30bd\u30fc\u30b9\u30c6\u30ad\u30b9\u30c8\u3092\u7de8\u96c6\u3057\u307e\u3059 ] \u7b49\u6e29\u306eDNA\u5897\u5e45\u3001\u4f8b\u3048\u3070B.\u30de\u30eb\u30c1\u30c7\u30a3\u30b9\u30d7\u30ec\u30fc\u30b9\u30e1\u30f3\u30c8\u5897\u5e45\u3001\u30ea\u30b3\u30f3\u30d3\u30ca\u30fc\u30bc\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u5897\u5e45\u307e\u305f\u306f\u7b49\u6e29\u30a2\u30bb\u30f3\u30d6\u30ea\u306f\u3001DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3092\u4f7f\u7528\u3057\u305f\u30b2\u30ce\u30e0\u5168\u4f53\u306e\u5897\u5e45\uff08\u4f8b\uff1a\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u306e\u03c629DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc PHI29 \uff09\u30b5\u30fc\u30de\u30ea\u30fc\u30b9\u30c8\u30c3\u30d7\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002 T4  – \u3001 T6 – \u305d\u3057\u3066\u305d\u306e T7 -DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3082\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30eb\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002 \u4f7f\u7528\u3055\u308c\u308b\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u9078\u629e\u306b\u52a0\u3048\u3066\u3001PCR\u306e\u6700\u9069\u5316\u306e\u904e\u7a0b\u3067PCR\u306e\u3055\u3089\u306a\u308b\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u304c\u7279\u306b\u5909\u66f4\u3055\u308c\u307e\u3059\u3002 PCR\u306b\u52a0\u3048\u3066\u3001\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001RT-PCR\u306e\u30d0\u30ea\u30a2\u30f3\u30c8\u3001\u7570\u306a\u308b\u30d0\u30ea\u30a2\u30f3\u30c8\u306eQPCR\u3001\u5c40\u6240\u7279\u7570\u7684\u5909\u7570\u8a98\u767a\u3001\u304a\u3088\u3073DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3082\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u3068\u30ce\u30fc\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u30d7\u30ed\u30fc\u30d6\u3082\u30e9\u30f3\u30c0\u30e0\u30d7\u30e9\u30a4\u30df\u30f3\u30b0\u306b\u3088\u3063\u3066\u751f\u6210\u3055\u308c\u307e\u3059\u3002 5 ‘\u21923’-\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027 \u30cb\u30c3\u30af\u7ffb\u8a33\u3084\u30bf\u30af\u30de\u30f3\u306a\u3069\u3001DNA\u306e\u5897\u52a0\uff08\u5897\u5e45\uff09\u306a\u3057\u3067\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002 \u2191 a b c d  J. Cline\u3001J\u3002C\u3002Braman\u3001H\u3002H\u3002Hogrefe\uff1a PFU DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304a\u3088\u3073\u305d\u306e\u4ed6\u306e\u71b1\u5b89\u5b9a\u6027DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306ePCR\u5fe0\u5b9f\u5ea6\u3002 \u306e\uff1a \u6838\u9178res\u3002 \u3001bd\u3002 24\u3001nr\u3002 18\u30011996\u3001S\u30023546\u20133551\u3002 PMID 8836181 ; PMC 146123 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191 a b  B.\u30b9\u30bf\u30fc\u30dd\u30d7\u30e9\u30f3\u30c9\u3001H\u3002\u30b9\u30fc\u30d6\u3001B\u3002\u30d5\u30e9\u30a4\u3001D\u3002\u30b7\u30f3\u30d6\u30eb\u30af\uff1a \u30c9\u30e1\u30a4\u30f3\u4ea4\u63db\uff1aThermus aquaticus DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3001\u5927\u8178\u83ccDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\u304a\u3088\u3073Thermotoga neapolitana DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u30ad\u30e1\u30e9\u3002 \u306e\uff1a \u30bf\u30f3\u30d1\u30af\u8cea1\u3002 \u3001bd\u3002 13\u3001nr\u3002 9\u30012000\u3001S\u3002645\u2013654\u3002 PMID 11054459 \u3002  \u2191  W. Abu Al-Soud\u3001P\u3002R\u00e2dstr\u00f6m\uff1a PCR\u963b\u5bb3\u30b5\u30f3\u30d7\u30eb\u306e\u5b58\u5728\u4e0b\u3067DNA\u5897\u5e45\u3092\u5a92\u4ecb\u3059\u308b9\u3064\u306e\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30ebDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5bb9\u91cf\u3002 \u306e\uff1a Appl\u3002\u74b0\u5883\u3002\u30de\u30a4\u30af\u30ed\u30d3\u30aa\u30fc\u30eb\u3002 \u3001bd\u3002 64\u3001nr\u3002 10\u30011998\u3001S\u30023748\u20133753\u3002 PMID 9758794 ; PMC 106538 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191  M. Takagi, M. Nishioka, H. Kakihara, M. Kitabayashi, H. Inoue, B. Kawakami, M. Oka, T. Imanaka: Pyrococcus sp\u3002\u304b\u3089\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u7279\u6027\u8a55\u4fa1KOD1\u682a\u3068PCR\u3078\u306e\u9069\u7528\u3002 \u306e\uff1a Appl\u3002\u74b0\u5883\u3002\u30de\u30a4\u30af\u30ed\u30d3\u30aa\u30fc\u30eb\u3002 \u3001bd\u3002 63\u3001nr\u3002 11\u30011997\u3001S\u30024504\u20134510\u3002 PMID 9361436 ; PMC 168769 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191  H. 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Wang\u3001D\u3002E\u3002Prosen\u3001L\u3002Mei\u3001J\u3002C\u3002Sullivan\u3001M\u3002Finney\u3001P\u3002B\u3002Vander Horn\uff1ain vitro\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u6027\u306e\u5411\u4e0a\u3068\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u306e\u5411\u4e0a\u306e\u305f\u3081\u306bDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3092\u8a2d\u8a08\u3059\u308b\u305f\u3081\u306e\u65b0\u3057\u3044\u6226\u7565\u3002\u306e\uff1a \u6838\u9178res\u3002 \uff082004\uff09\u3001bd\u3002 32\uff083\uff09\u3001S\u30021197\u2013207\u3002 PMID 14973201 ; PMC 373405 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191  M. 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Barnes\uff1aLambda Bacteriophage\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u304b\u3089\u306e\u9ad8\u3044\u5fe0\u5b9f\u5ea6\u3068\u9ad8\u53ce\u91cf\u306e\u6700\u592735 kb DNA\u306ePCR\u5897\u5e45\u3002\u306e\uff1a Proc Natl Acad Sci U S a \uff081994\uff09\u3001bd\u3002 91\uff086\uff09\u3001S\u30022216\u201320\u3002 PMID 8134376 ; PMC 43341 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191  \u7279\u8a31 EP1752534A1 \uff1a \u9ad8\u901fDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3092\u4f7f\u7528\u3057\u305f\u9ad8\u901fPCR\u3002 \u767b\u9332 12. 2005\u5e745\u6708 \u3001\u30aa\u30f3\u306b\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059 2007\u5e742\u670814\u65e5 \u3001Ampelder\uff1aToyo Boseki\u3001Erfinder\uff1aMasaya Segawa et al\u3002 \u200c  \u2191  \u7279\u8a31 US2013034879A1 \uff1a DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3002 \u767b\u9332 2. 2012\u5e748\u6708 \u3001\u30aa\u30f3\u306b\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059 2007\u5e742\u670814\u65e5 \u3001Anmelder\uff1aEnzyme Uab et al\u3001Erfinder\uff1aRemigijus skirgaila et al\u3002 \u200c  \u2191  \u7279\u8a31 US2009280539A1 \uff1a DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304a\u3088\u3073\u95a2\u9023\u3059\u308b\u65b9\u6cd5\u3002 \u767b\u9332 16. 2009\u5e744\u6708 \u3001\u30aa\u30f3\u306b\u516c\u958b\u3055\u308c\u3066\u3044\u307e\u3059 12. 2009\u5e7411\u6708 \u3001\u767b\u9332\uff1aRoche Molecular Systems Inc\u3001\u767a\u660e\u5bb6\uff1a\u30ad\u30fc\u30b9A.\u30d0\u30a6\u30a2\u30fc\u3002 \u200c  \u2191  W. M.\u30d0\u30fc\u30f3\u30ba\uff1a PCR\u3092\u89e6\u5a92\u3059\u308bTAQ\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5fe0\u5b9f\u5ea6\u306f\u3001N\u672b\u7aef\u306e\u6b20\u5931\u306b\u3088\u3063\u3066\u6539\u5584\u3055\u308c\u307e\u3059 \u3002\u306e\uff1a \u907a\u4f1d\u5b50 \uff081992\uff09\u3001\u30d0\u30f3\u30c9112\uff081\uff09\u3001S\u300229\u201335\u3002 PMID 1551596 \u3002  \u2191  L. S.\u30e1\u30eb\u30b1\u30f3\u30ba\u3001S\u3002K\u3002\u30d6\u30e9\u30a4\u30a2\u30f3\u3001R\u3002E\u3002\u30e2\u30fc\u30bc\u30b9\uff1a Thermus aquaticus DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e5′-3 ‘\u30a8\u30ad\u30bd\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306e\u4e0d\u6d3b\u6027\u5316\u3002 \u306e\uff1a Biochim Biophys Acta \uff081995\uff09\u3001\u30d0\u30f3\u30c91264\uff082\uff09\u3001S\u3002243\u20138\u3002 PMID 7495870 \u3002  \u2191  S.\u30bf\u30dc\u30fc\u30eb\u3001C\u3002C\u3002\u30ea\u30c1\u30e3\u30fc\u30c9\u30bd\u30f3\uff1a \u5927\u8178\u83ccDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bcI\u30d5\u30a1\u30df\u30ea\u30fc\u306eDNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u5358\u4e00\u6b8b\u57fa\u306f\u3001\u30c7\u30aa\u30ad\u30b7\u3068\u30b8\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3092\u533a\u5225\u3059\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3059\u3002 \u306e\uff1a Proc Natl Acad Sci U S A. \u3001\u30d0\u30f3\u30c992\u3001nr\u3002 14\u30011995\u3001S\u30026339-6343\u3002 PMID 7603992 ; PMC 41513 \uff08\u7121\u6599\u306e\u5168\u6587\uff09\u3002  \u2191  P. B. Vander Horn\u3001M\u3002C\u3002Davis\u3001J\u3002J\u3002Cunniff\u3001C\u3002Ruan\u3001B\u3002F\u3002McArdle\u3001S\u3002B\u3002Samols\u3001J\u3002Szasz\u3001G\u3002Hu\u3001K\u3002M\u3002Hujer\u3001S\u3002T\u3002Domke\u3001S\u3002R\u3002Brummet\u3001R\u3002B\u3002Moffett\u3001C\u3002W\u3002Fuller\uff1a Thermo Sequenase DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304a\u3088\u3073T.\u9178\u6027\u75be\u60a3\u30d4\u30ed\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\uff1aDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u305f\u3081\u306e\u65b0\u3057\u3044\u30b5\u30fc\u30e2\u30b9\u30bf\u30d6\u30eb\u9175\u7d20\u3002 \u306e\uff1a \u30d0\u30a4\u30aa\u30c6\u30af\u30cb\u30c3\u30af \u3001\u30d0\u30f3\u30c922\u3001nr\u3002 4\u30011997\u3001S\u3002758-172\u3001764-765\u3002 PMID 9105629 \u3002  \u2191  J. M. Prober\u3001G\u3002L\u3002Trainor\u3001R\u3002J\u3002Dam\u3001F\u3002W\u3002Hobbs\u3001C\u3002W\u3002Robertson\u3001R\u3002J\u3002Zagursky\u3001A\u3002J\u3002Cocuzza\u3001M\u3002A\u3002Jensen\u3001K\u3002Baumeister\uff1a \u86cd\u5149\u9396\u30bf\u30fc\u30df\u30cd\u30fc\u30c8\u30b8\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3092\u4f7f\u7528\u3057\u305f\u8fc5\u901f\u306aDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30b7\u30b9\u30c6\u30e0\u3002 \u306e\uff1a \u5316\u5b66 \u3001\u30d0\u30f3\u30c9238\u3001nr\u3002 4825\u30011987\u3001S\u3002336-341\u3002 PMID 2443975 \u3002       (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4"},{"@context":"http:\/\/schema.org\/","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"item":{"@id":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/#breadcrumbitem","name":"Enzyklop\u00e4die"}},{"@type":"ListItem","position":2,"item":{"@id":"https:\/\/wiki.edu.vn\/all2jp\/wiki10\/archives\/16999#breadcrumbitem","name":"\u30b5\u30fc\u30e2\u30b9\u30bf\u30d3\u30ebDNA-\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc – \u30a6\u30a3\u30ad\u30da\u30c7\u30a3\u30a2"}}]}]