[{"@context":"http:\/\/schema.org\/","@type":"BlogPosting","@id":"https:\/\/wiki.edu.vn\/en\/wiki24\/methylmalonyl-coa-mutase-wikipedia\/#BlogPosting","mainEntityOfPage":"https:\/\/wiki.edu.vn\/en\/wiki24\/methylmalonyl-coa-mutase-wikipedia\/","headline":"Methylmalonyl-CoA mutase &#8211; Wikipedia","name":"Methylmalonyl-CoA mutase &#8211; Wikipedia","description":"before-content-x4 Mammalian protein found in Homo sapiens after-content-x4 Methylmalonyl-CoA mutase (EC 5.4.99.2, MCM), mitochondrial, also known as methylmalonyl-CoA isomerase, is","datePublished":"2016-09-25","dateModified":"2016-09-25","author":{"@type":"Person","@id":"https:\/\/wiki.edu.vn\/en\/wiki24\/author\/lordneo\/#Person","name":"lordneo","url":"https:\/\/wiki.edu.vn\/en\/wiki24\/author\/lordneo\/","image":{"@type":"ImageObject","@id":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","height":96,"width":96}},"publisher":{"@type":"Organization","name":"Enzyklop\u00e4die","logo":{"@type":"ImageObject","@id":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/08\/download.jpg","url":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/08\/download.jpg","width":600,"height":60}},"image":{"@type":"ImageObject","@id":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/e\/eb\/MUT.png\/490px-MUT.png","url":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/e\/eb\/MUT.png\/490px-MUT.png","height":"383","width":"490"},"url":"https:\/\/wiki.edu.vn\/en\/wiki24\/methylmalonyl-coa-mutase-wikipedia\/","wordCount":11458,"articleBody":"     (adsbygoogle = window.adsbygoogle || []).push({});before-content-x4Mammalian protein found in Homo sapiens       (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4Methylmalonyl-CoA mutase (EC 5.4.99.2, MCM), mitochondrial, also known as methylmalonyl-CoA isomerase, is a protein that in humans is encoded by the MUT gene. This vitamin B12-dependent enzyme catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA in humans. Mutations in MUT gene may lead to various types of methylmalonic aciduria.[5]MCM was first identified in rat liver and sheep kidney in 1955. In its latent form, it is 750 amino acids in length. Upon entry to the mitochondria, the 32 amino acid mitochondrial leader sequence at the N-terminus of the protein is cleaved, forming the fully processed monomer. The monomers then associate into homodimers, and bind AdoCbl (one for each monomer active site) to form the final, active holoenzyme form.[6]       (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4Table of ContentsStructure[edit]Gene[edit]Protein[edit]Function[edit]Clinical significance[edit]Mechanism[edit]Interactions[edit]References[edit]Further reading[edit]External links[edit]Structure[edit]Gene[edit]The MUT gene lies on the chromosome location of 6p12.3 and consists of 13 exons, spanning over 35kb.[7]Protein[edit]The mature enzyme is a homodimer with the N-terminal CoA binding domain and the C- terminal cobalamin-binding domain.[8]Function[edit]Methylmalonyl-CoA mutase is expressed in high concentrations in the kidney, in intermediate concentrations in the heart, ovaries, brain, muscle, and liver, and in low concentrations in the spleen.[6] The enzyme can be found all throughout the central nervous system (CNS).[6] MCM resides in the mitochondria, where a number of substances, including the branched-chain amino acids isoleucine and valine, as well as methionine, threonine, thymine and odd-chain fatty acids, are metabolized via methylmalonate semialdehyde (MMlSA) or propionyl-CoA (Pr-CoA) to a common compound &#8211; methylmalonyl-CoA (MMl-CoA).MCM catalyzes the reversible isomerisation of l\u2010methylmalonyl\u2010CoA to succinyl\u2010CoA, requiring cobalamin (vitamin B12) in the form of adenosylcobalamin (AdoCbl) as a cofactor. As an important step in propionate catabolism, this reaction is required for the degradation of odd-chain fatty acids, the amino acids valine, isoleucine, methionine, and threonine, and cholesterol,[9] funneling metabolites from the breakdown of these amino acids into the tricarboxylic acid cycle.[10]       (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4Methylmalonyl-CoA mutase catalyzes the following reaction:The substrate of methylmalonyl-CoA mutase, methylmalonyl-CoA, is primarily derived from  propionyl-CoA, a substance formed from the catabolism and digestion of isoleucine, valine, threonine, methionine, thymine, cholesterol, or odd-chain fatty acids. The product of the enzyme, succinyl-CoA, is a key molecule of the tricarboxylic acid cycle.Clinical significance[edit]A deficiency of this enzyme is responsible for an inherited disorder of metabolism, methylmalonyl-CoA mutase deficiency, which is one of the causes of methylmalonic acidemia (also referred to as methylmalonic aciduria or MMA). MMA is an autosomal recessive inherited inborn error of metabolism, characterized by recurrent episodes of vomiting, lethargy, profound ketoacidosis, hyperammonemia, and pancytopenia in infancy, and may cause early death. Complications include cardiomyopathy, metabolic stroke, pancreatitis, and progressive renal failure.[5][11]Either mutations to the gene MUT (encodes methylmalonyl-CoA mutase), or MMAA (encodes a chaperone protein of methylmalonyl-CoA mutase, MMAA protein) can lead to methylmalonyl acidemia.[12] Mutations to MUT can be categorized as either MUT0 (demonstrates no activity even in presence of excess AdoCbl), or MUT1 (demonstrates very low activity in presence of excess AdoCbl).[8] Over half of the mutations of MUT are missense mutations[10] while nonsense mutations comprise a significant remaining fraction (approximately 14%)[13]Common treatment methods for MMA include a liver transplant or a liver and kidney transplant to combat the renal disease of methylmalonic acidemia. However, detrimental neurological effects can continue to plague patients even after a successful operation. It is thought that this is due to the widespread presence of methylmalonyl-CoA mutase throughout the central nervous system. Due to the loss of functionality of the enzyme, substrate levels build up in the CNS. The substrate, L-methylmalonyl-CoA hydrolyzes to form methylmalonate (methylmalonic acid), a neurotoxic dicarboxylic acid that, due to the poor dicarboxylic acid transport capacities of the blood-brain barrier, is effectively trapped within the CNS, leading to neurological debilitation. To combat these effects perioperative anti-catabolic regimes and no diet discontinuation are recommended.[6]The murine model has proven an adequate and accurate way of studying the effects of MMA, and potential treatment methods.[14][15]Mechanism[edit]  The MCM reaction mechanism begins with homolytic cleavage of AdoB12&#8217;s C-Co(III) bond, the C and Co atoms each acquire one of the electrons that formed the cleaved electron pair bond. The Co ion, therefore, fluctuates between its Co(III) and Co(II) oxidation states [the two states are spectroscopically distinguishable: Co(III) is red and diamagnetic (no unpaired electrons), whereas Co(II) is yellow and paramagnetic (unpaired electrons)]. Hence, the role of coenzyme B-12 in the catalytic process is that of a reversible generator of a free radical. The C-Co(III) bond is weak, with a dissociation energy = 109 kJ\/mol, and appears to be further weakened through steric interactions with the enzyme. The homolytic reaction is unusual in biology, as is the presence of a metal-carbon bond.Methylmalonyl-CoA mutase is a member of the isomerase subfamily of adenosylcobalamin-dependent enzymes. Furthermore, it is classified as class I, as it is a \u2018DMB-off\u2019\/\u2019His-on\u2019 enzyme. This refers to the nature of the AdoCbl cofactor in the active site of methylmalonyl CoA.[16] AdoCbl is composed of a central cobalt-containing corrin ring, an upper axial ligand (\u03b2-axial ligand), and a lower axial ligand (\u03b1-axial ligand). In methylmalonyl-CoA mutase, the \u03b2-axial ligand 5\u2019-deoxy-5\u2019-adenosine reversibly dissociated to give the deoxyadenosyl radical. The \u03b1-axial ligand 5,6-dimethylbenzimidazole (DMB) is involved in organizing the active site to enable histidine-610 to bond with Co, instead of DMB (the reason for the \u2018DMB-off\u2019\/\u2019His-on\u2019 notation).[16] Binding of histidine-610 residue increases the rate of homolytic \u03b2-axial ligand \u2013 Co bond breakage by a factor of 1012.[17]  MCM active site. Corrin ring and \u03b1-axial ligand (DMB): (yellow), \u03b2-axial ligand: (green), substrate\/product: (cyan), residues interacting with \u03b2-axial ligand: glu370, asn366, gly91, ala139 (blue), residues interacting with substrate: gln197, his244, arg207, tyr89 (red), and his610: (orange).[18] Rendered from PDB 4REQ.[19]Other important residues of methylmalonyl-CoA mutase include Histidine-244, which acts as a general acid near the substrate and shields the radical species from side reactions involving oxygen,[20]Glutamate-370, whose hydrogen bond with the 2\u2019-OH group of the ribose of the \u03b2-axial ligand forces interaction between the \u03b2-axial ligand radical species and the substrate,[21] and tyrosine-89 which stabilizes reactive radical intermediates and accounts for the stereo-selectivity of the enzyme.[18][22]The processing protein, MMAA protein, fills the important role of aiding cofactor loading and exchange.[12][23]MMAA protein favors association with the MCM apoenzyme, and allows for the transfer of the AdoCbl cofactor to the enzyme active site.[23] Furthermore, if the bound AdoCbl accrues oxidative damage during normal functioning, MMAA protein fosters exchange of the damaged cofactor for a new AdoCbl via a GTP-reliant pathway.[12][23]Interactions[edit]References[edit]^ a b c GRCh38: Ensembl release 89: ENSG00000146085 &#8211; Ensembl, May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000023921 &#8211; Ensembl, May 2017^ &#8220;Human PubMed Reference:&#8221;. National Center for Biotechnology Information, U.S. National Library of Medicine.^ &#8220;Mouse PubMed Reference:&#8221;. National Center for Biotechnology Information, U.S. National Library of Medicine.^ a b Keyfi F, Sankian M, Moghaddassian M, Rolfs A, Varasteh AR (January 2016). &#8220;Molecular, biochemical, and structural analysis of a novel mutation in patients with methylmalonyl-CoA mutase deficiency&#8221;. Gene. 576 (1 Pt 2): 208\u201313. doi:10.1016\/j.gene.2015.10.002. PMID\u00a026449400.^ a b c d Ballhausen D, Mittaz L, Boulat O, Bonaf\u00e9 L, Braissant O (December 2009). &#8220;Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain&#8221;. Neuroscience. 164 (2): 578\u201387. doi:10.1016\/j.neuroscience.2009.08.028. PMID\u00a019699272. S2CID\u00a034612963.^ Chandler RJ, Venditti CP (2016-10-01). &#8220;Genetic and genomic systems to study methylmalonic acidemia&#8221;. Molecular Genetics and Metabolism. 86 (1\u20132): 34\u201343. doi:10.1016\/j.ymgme.2005.07.020. PMC\u00a02657357. PMID\u00a016182581.^ a b Drennan CL, Matthews RG, Rosenblatt DS, Ledley FD, Fenton WA, Ludwig ML (May 1996). &#8220;Molecular basis for dysfunction of some mutant forms of methylmalonyl-CoA mutase: deductions from the structure of methionine synthase&#8221;. Proceedings of the National Academy of Sciences of the United States of America. 93 (11): 5550\u20135. Bibcode:1996PNAS&#8230;93.5550D. doi:10.1073\/pnas.93.11.5550. PMC\u00a039284. PMID\u00a08643613.^ Mart\u00ednez MA, Rinc\u00f3n A, Desviat LR, Merinero B, Ugarte M, P\u00e9rez B (April 2005). &#8220;Genetic analysis of three genes causing isolated methylmalonic acidemia: identification of 21 novel allelic variants&#8221;. Molecular Genetics and Metabolism. 84 (4): 317\u201325. doi:10.1016\/j.ymgme.2004.11.011. PMID\u00a015781192.^ a b Forny P, Froese DS, Suormala T, Yue WW, Baumgartner MR (December 2014). &#8220;Functional characterization and categorization of missense mutations that cause methylmalonyl-CoA mutase (MUT) deficiency&#8221;. Human Mutation. 35 (12): 1449\u201358. doi:10.1002\/humu.22633. PMC\u00a04441004. PMID\u00a025125334.^ D\u00fcndar H, \u00d6zg\u00fcl RK, G\u00fczel-Ozant\u00fcrk A, Dursun A, Sivri S, Aliefendio\u011flu D, Co\u015fkun T, Tokatli A (August 2012). &#8220;Microarray based mutational analysis of patients with methylmalonic acidemia: identification of 10 novel mutations&#8221;. Molecular Genetics and Metabolism. 106 (4): 419\u201323. doi:10.1016\/j.ymgme.2012.05.014. PMID\u00a022727635.^ a b c Takahashi-\u00cd\u00f1iguez T, Garc\u00eda-Arellano H, Trujillo-Rold\u00e1n MA, Flores ME (January 2011). &#8220;Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein&#8221;. Biochemical and Biophysical Research Communications. 404 (1): 443\u20137. doi:10.1016\/j.bbrc.2010.11.141. PMID\u00a021138732.^ Buck NE, Wood LR, Hamilton NJ, Bennett MJ, Peters HL (November 2012). &#8220;Treatment of a methylmalonyl-CoA mutase stopcodon mutation&#8221;. Biochemical and Biophysical Research Communications. 427 (4): 753\u20137. doi:10.1016\/j.bbrc.2012.09.133. PMID\u00a023041189.^ Chandler RJ, Venditti CP (November 2012). &#8220;Pre-clinical efficacy and dosing of an AAV8 vector expressing human methylmalonyl-CoA mutase in a murine model of methylmalonic acidemia (MMA)&#8221;. Molecular Genetics and Metabolism. 107 (3): 617\u20139. doi:10.1016\/j.ymgme.2012.09.019. PMC\u00a03522145. PMID\u00a023046887.^ Manoli I, Sysol JR, Li L, Houillier P, Garone C, Wang C, Zerfas PM, Cusmano-Ozog K, Young S, Trivedi NS, Cheng J, Sloan JL, Chandler RJ, Abu-Asab M, Tsokos M, Elkahloun AG, Rosen S, Enns GM, Berry GT, Hoffmann V, DiMauro S, Schnermann J, Venditti CP (August 2013). &#8220;Targeting proximal tubule mitochondrial dysfunction attenuates the renal disease of methylmalonic acidemia&#8221;. Proceedings of the National Academy of Sciences of the United States of America. 110 (33): 13552\u20137. Bibcode:2013PNAS..11013552M. doi:10.1073\/pnas.1302764110. PMC\u00a03746875. PMID\u00a023898205.^ a b Takahashi-I\u00f1iguez T, Garc\u00eda-Hernandez E, Arregu\u00edn-Espinosa R, Flores ME (June 2012). &#8220;Role of vitamin B12 on methylmalonyl-CoA mutase activity&#8221;. Journal of Zhejiang University Science B. 13 (6): 423\u201337. doi:10.1631\/jzus.B1100329. PMC\u00a03370288. PMID\u00a022661206.^ Vlasie M, Chowdhury S, Banerjee R (May 2002). &#8220;Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutase&#8221;. The Journal of Biological Chemistry. 277 (21): 18523\u20137. doi:10.1074\/jbc.M111809200. PMID\u00a011893736.^ a b Mancia F, Smith GA, Evans PR (June 1999). &#8220;Crystal structure of substrate complexes of methylmalonyl-CoA mutase&#8221;. Biochemistry. 38 (25): 7999\u20138005. doi:10.1021\/bi9903852. PMID\u00a010387043.^ Mancia F, Evans PR (June 1998). &#8220;Conformational changes on substrate binding to methylmalonyl CoA mutase and new insights into the free radical mechanism&#8221;. Structure. 6 (6): 711\u201320. doi:10.1016\/s0969-2126(98)00073-2. PMID\u00a09655823.^ Maiti N, Widjaja L, Banerjee R (November 1999). &#8220;Proton transfer from histidine 244 may facilitate the 1,2 rearrangement reaction in coenzyme B(12)-dependent methylmalonyl-CoA mutase&#8221;. The Journal of Biological Chemistry. 274 (46): 32733\u20137. doi:10.1074\/jbc.274.46.32733. PMID\u00a010551831.^ Buckel W, Friedrich P, Golding BT (October 2012). &#8220;Hydrogen bonds guide the short-lived 5&#8242;-deoxyadenosyl radical to the place of action&#8221;. Angewandte Chemie. 51 (40): 9974\u20136. doi:10.1002\/anie.201205299. PMID\u00a022945861.^ Thom\u00e4 NH, Meier TW, Evans PR, Leadlay PF (October 1998). &#8220;Stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-CoA mutase&#8221;. Biochemistry. 37 (41): 14386\u201393. CiteSeerX\u00a010.1.1.608.304. doi:10.1021\/bi981375o. PMID\u00a09772164.^ a b c d e Froese DS, Kochan G, Muniz JR, Wu X, Gileadi C, Ugochukwu E, Krysztofinska E, Gravel RA, Oppermann U, Yue WW (December 2010). &#8220;Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation&#8221;. The Journal of Biological Chemistry. 285 (49): 38204\u201313. doi:10.1074\/jbc.M110.177717. PMC\u00a02992254. PMID\u00a020876572.^ Padovani D, Labunska T, Banerjee R (June 2006). &#8220;Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase&#8221;. The Journal of Biological Chemistry. 281 (26): 17838\u201344. doi:10.1074\/jbc.M600047200. PMID\u00a016641088.^ Taoka S, Padmakumar R, Lai MT, Liu HW, Banerjee R (December 1994). &#8220;Inhibition of the human methylmalonyl-CoA mutase by various CoA-esters&#8221;. The Journal of Biological Chemistry. 269 (50): 31630\u20134. doi:10.1016\/S0021-9258(18)31741-1. PMID\u00a07989334.Further reading[edit]Ledley FD, Rosenblatt DS (1997). &#8220;Mutations in mut methylmalonic acidemia: clinical and enzymatic correlations&#8221;. Human Mutation. 9 (1): 1\u20136. doi:10.1002\/(SICI)1098-1004(1997)9:13.0.CO;2-E. PMID\u00a08990001. S2CID\u00a041661834.Ludwig ML, Matthews RG (1997). &#8220;Structure-based perspectives on B12-dependent enzymes&#8221;. Annual Review of Biochemistry. 66: 269\u2013313. doi:10.1146\/annurev.biochem.66.1.269. PMID\u00a09242908.Lubrano R, Elli M, Rossi M, Travasso E, Raggi C, Barsotti P, Carducci C, Berloco P (August 2007). &#8220;Renal transplant in methylmalonic acidemia: could it be the best option? Report on a case at 10 years and review of the literature&#8221;. Pediatric Nephrology. 22 (8): 1209\u201314. doi:10.1007\/s00467-007-0460-z. PMID\u00a017401587. S2CID\u00a024610554.Frenkel EP, Kitchens RL (December 1975). &#8220;Intracellular localization of hepatic propionyl-CoA carboxylase and methylmalonyl-CoA mutase in humans and normal and vitamin B12 deficient rats&#8221;. British Journal of Haematology. 31 (4): 501\u201313. doi:10.1111\/j.1365-2141.1975.tb00885.x. PMID\u00a024458. S2CID\u00a01232083.Crane AM, Jansen R, Andrews ER, Ledley FD (February 1992). &#8220;Cloning and expression of a mutant methylmalonyl coenzyme A mutase with altered cobalamin affinity that causes mut- methylmalonic aciduria&#8221;. The Journal of Clinical Investigation. 89 (2): 385\u201391. doi:10.1172\/JCI115597. PMC\u00a0442864. PMID\u00a01346616.Crane AM, Martin LS, Valle D, Ledley FD (May 1992). &#8220;Phenotype of disease in three patients with identical mutations in methylmalonyl CoA mutase&#8221;. Human Genetics. 89 (3): 259\u201364. doi:10.1007\/BF00220536. PMID\u00a01351030. S2CID\u00a05624280.Raff ML, Crane AM, Jansen R, Ledley FD, Rosenblatt DS (January 1991). &#8220;Genetic characterization of a MUT locus mutation discriminating heterogeneity in mut0 and mut- methylmalonic aciduria by interallelic complementation&#8221;. The Journal of Clinical Investigation. 87 (1): 203\u20137. doi:10.1172\/JCI114972. PMC\u00a0295026. PMID\u00a01670635.Jansen R, Ledley FD (November 1990). &#8220;Heterozygous mutations at the mut locus in fibroblasts with mut0 methylmalonic acidemia identified by polymerase-chain-reaction cDNA cloning&#8221;. American Journal of Human Genetics. 47 (5): 808\u201314. PMC\u00a01683687. PMID\u00a01977311.Nham SU, Wilkemeyer MF, Ledley FD (December 1990). &#8220;Structure of the human methylmalonyl-CoA mutase (MUT) locus&#8221;. Genomics. 8 (4): 710\u20136. doi:10.1016\/0888-7543(90)90259-W. PMID\u00a01980486.Ledley FD, Lumetta M, Nguyen PN, Kolhouse JF, Allen RH (May 1988). &#8220;Molecular cloning of L-methylmalonyl-CoA mutase: gene transfer and analysis of mut cell lines&#8221;. Proceedings of the National Academy of Sciences of the United States of America. 85 (10): 3518\u201321. Bibcode:1988PNAS&#8230;85.3518L. doi:10.1073\/pnas.85.10.3518. PMC\u00a0280243. PMID\u00a02453061.Jansen R, Kalousek F, Fenton WA, Rosenberg LE, Ledley FD (February 1989). &#8220;Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction&#8221;. Genomics. 4 (2): 198\u2013205. doi:10.1016\/0888-7543(89)90300-5. PMID\u00a02567699.Fenton WA, Hack AM, Kraus JP, Rosenberg LE (March 1987). &#8220;Immunochemical studies of fibroblasts from patients with methylmalonyl-CoA mutase apoenzyme deficiency: detection of a mutation interfering with mitochondrial import&#8221;. Proceedings of the National Academy of Sciences of the United States of America. 84 (5): 1421\u20134. Bibcode:1987PNAS&#8230;84.1421F. doi:10.1073\/pnas.84.5.1421. PMC\u00a0304442. PMID\u00a02881300.Zoghbi HY, O&#8217;Brien WE, Ledley FD (November 1988). &#8220;Linkage relationships of the human methylmalonyl CoA mutase to the HLA and D6S4 loci on chromosome 6&#8221;. Genomics. 3 (4): 396\u20138. doi:10.1016\/0888-7543(88)90135-8. PMID\u00a02907507.Kolhouse JF, Utley C, Allen RH (April 1980). &#8220;Isolation and characterization of methylmalonyl-CoA mutase from human placenta&#8221;. The Journal of Biological Chemistry. 255 (7): 2708\u201312. doi:10.1016\/S0021-9258(19)85795-2. PMID\u00a06102092.Fenton WA, Hack AM, Willard HF, Gertler A, Rosenberg LE (April 1982). &#8220;Purification and properties of methylmalonyl coenzyme A mutase from human liver&#8221;. Archives of Biochemistry and Biophysics. 214 (2): 815\u201323. doi:10.1016\/0003-9861(82)90088-1. PMID\u00a06124211.Qureshi AA, Crane AM, Matiaszuk NV, Rezvani I, Ledley FD, Rosenblatt DS (April 1994). &#8220;Cloning and expression of mutations demonstrating intragenic complementation in mut0 methylmalonic aciduria&#8221;. The Journal of Clinical Investigation. 93 (4): 1812\u20139. doi:10.1172\/JCI117166. PMC\u00a0294249. PMID\u00a07909321.Crane AM, Ledley FD (July 1994). &#8220;Clustering of mutations in methylmalonyl CoA mutase associated with mut- methylmalonic acidemia&#8221;. American Journal of Human Genetics. 55 (1): 42\u201350. PMC\u00a01918235. PMID\u00a07912889.Janata J, Kogekar N, Fenton WA (September 1997). &#8220;Expression and kinetic characterization of methylmalonyl-CoA mutase from patients with the mut- phenotype: evidence for naturally occurring interallelic complementation&#8221;. Human Molecular Genetics. 6 (9): 1457\u201364. doi:10.1093\/hmg\/6.9.1457. PMID\u00a09285782.External links[edit]     (adsbygoogle = window.adsbygoogle || []).push({});after-content-x4"},{"@context":"http:\/\/schema.org\/","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"item":{"@id":"https:\/\/wiki.edu.vn\/en\/wiki24\/#breadcrumbitem","name":"Enzyklop\u00e4die"}},{"@type":"ListItem","position":2,"item":{"@id":"https:\/\/wiki.edu.vn\/en\/wiki24\/methylmalonyl-coa-mutase-wikipedia\/#breadcrumbitem","name":"Methylmalonyl-CoA mutase &#8211; Wikipedia"}}]}]