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a b c Ellis RJ (October 2001). \u201cMacromolecular crowding: obvious but underappreciated\u201d. Trends Biochem. Sci. 26 (10):  597\u2013604. PMID\u00a011590012.\u00a0^ a b Zimmerman SB, Trach SO (December 1991). \u201cEstimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli\u201d. J. Mol. Biol. 222 (3):  599\u2013620. PMID\u00a01748995.\u00a0^ a b Minton AP (July 2006). \u201cHow can biochemical reactions within cells differ from those in test tubes?\u201d. J. Cell. Sci. 119 (Pt 14):  2863\u20139. doi:10.1242\/jcs.03063. PMID\u00a016825427. http:\/\/jcs.biologists.org\/cgi\/content\/full\/119\/14\/2863.\u00a0^ Kubitschek HE (January 1990). \u201cCell volume increase in Escherichia coli after shifts to richer media\u201d. J. Bacteriol. 172 (1):  94\u2013101. PMC\u00a0208405. PMID\u00a02403552. http:\/\/jb.asm.org\/cgi\/pmidlookup?view=long&pmid=2403552.\u00a0^ Blattner FR, Plunkett G, Bloch CA, et al (September 1997). \u201cThe complete genome sequence of Escherichia coli K-12\u201d. Science (journal) 277 (5331):  1453\u201374. PMID\u00a09278503. http:\/\/www.sciencemag.org\/cgi\/pmidlookup?view=long&pmid=9278503.\u00a0^ Han MJ, Lee SY (June 2006). \u201cThe Escherichia coli proteome: past, present, and future prospects\u201d. Microbiol. Mol. Biol. Rev. 70 (2):  362\u2013439. doi:10.1128\/MMBR.00036-05. PMC\u00a01489533. PMID\u00a016760308. http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?tool=pubmed&pubmedid=16760308.\u00a0^ Minton AP (October 1992). \u201cConfinement as a determinant of macromolecular structure and reactivity\u201d. Biophys. J. 63 (4):  1090\u2013100. PMC\u00a01262248. PMID\u00a01420928. http:\/\/www.biophysj.org\/cgi\/reprint\/63\/4\/1090.\u00a0^ a b c Minton AP (2001). \u201cThe influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media\u201d. J. Biol. Chem. 276 (14):  10577\u201380. doi:10.1074\/jbc.R100005200. PMID\u00a011279227. http:\/\/www.jbc.org\/cgi\/content\/full\/276\/14\/10577.\u00a0^ Zhou HX, Rivas G, Minton AP (2008). \u201cMacromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences\u201d. Annu Rev Biophys 37:  375\u201397. doi:10.1146\/annurev.biophys.37.032807.125817. PMID\u00a018573087.\u00a0^ Zimmerman SB (November 1993). \u201cMacromolecular crowding effects on macromolecular interactions: some implications for genome structure and function\u201d. Biochim. Biophys. Acta 1216 (2):  175\u201385. PMID\u00a08241257.\u00a0^ Zimmerman SB, Harrison B (April 1987). \u201cMacromolecular crowding increases binding of DNA polymerase to DNA: an adaptive effect\u201d. Proc. Natl. Acad. Sci. U.S.A. 84 (7):  1871\u20135. PMC\u00a0304543. PMID\u00a03550799. http:\/\/www.pnas.org\/cgi\/pmidlookup?view=long&pmid=3550799.\u00a0^ van den Berg B, Wain R, Dobson CM, Ellis RJ (August 2000). \u201cMacromolecular crowding perturbs protein refolding kinetics: implications for folding inside the cell\u201d. EMBO J. 19 (15):  3870\u20135. doi:10.1093\/emboj\/19.15.3870. PMC\u00a0306593. PMID\u00a010921869. https:\/\/doi.org\/10.1093\/emboj\/19.15.3870.\u00a0^ van den Berg B, Ellis RJ, Dobson CM (December 1999). \u201cEffects of macromolecular crowding on protein folding and aggregation\u201d. EMBO J. 18 (24):  6927\u201333. doi:10.1093\/emboj\/18.24.6927. PMC\u00a01171756. PMID\u00a010601015. https:\/\/doi.org\/10.1093\/emboj\/18.24.6927.\u00a0^ Ellis RJ, Minton AP (May 2006). \u201cProtein aggregation in crowded environments\u201d. Biol. Chem. 387 (5):  485\u201397. doi:10.1515\/BC.2006.064. PMID\u00a016740119.\u00a0^ Martin J, Hartl FU (February 1997). \u201cThe effect of macromolecular crowding on chaperonin-mediated protein folding\u201d. Proc. Natl. Acad. Sci. U.S.A. 94 (4):  1107\u201312. PMC\u00a019752. PMID\u00a09037014. http:\/\/www.pnas.org\/cgi\/pmidlookup?view=long&pmid=9037014.\u00a0^ Ellis RJ (2007). \u201cProtein misassembly: macromolecular crowding and molecular chaperones\u201d. Adv. Exp. Med. Biol. 594:  1\u201313. PMID\u00a017205670.\u00a0^ Benedek GB (September 1997). \u201cCataract as a protein condensation disease: the Proctor Lecture\u201d. Invest. Ophthalmol. Vis. Sci. 38 (10):  1911\u201321. PMID\u00a09331254. http:\/\/www.iovs.org\/cgi\/reprint\/38\/10\/1911.\u00a0^ Steadman BL, Trautman PA, Lawson EQ, et al (December 1989). \u201cA differential scanning calorimetric study of the bovine lens crystallins\u201d. Biochemistry 28 (25):  9653\u20138. PMID\u00a02611254.\u00a0^ Bloemendal H, de Jong W, Jaenicke R, Lubsen NH, Slingsby C, Tardieu A (November 2004). \u201cAgeing and vision: structure, stability and function of lens crystallins\u201d. Prog. Biophys. Mol. Biol. 86 (3):  407\u201385. doi:10.1016\/j.pbiomolbio.2003.11.012. PMID\u00a015302206.\u00a0^ Tokuriki N, Kinjo M, Negi S, et al (January 2004). \u201cProtein folding by the effects of macromolecular crowding\u201d. Protein Sci. 13 (1):  125\u201333. PMC\u00a02286514. 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