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[{"@context":"http:\/\/schema.org\/","@type":"BlogPosting","@id":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/3550#BlogPosting","mainEntityOfPage":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/3550","headline":"\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc – Wikipedia","name":"\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc – Wikipedia","description":"\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\uff08Aryldialkylphosphatase\u3001EC 3.1.8.1\uff09\u306f\u3001 \u6709\u6a5f\u30ea\u30f3\u7cfb\u6bba\u866b\u5264\u306e\u30ea\u30f3\u9178\u30c8\u30ea\u30a8\u30b9\u30c6\u30eb\u306e\u52a0\u6c34\u5206\u89e3\u3092\u89e6\u5a92\u3059\u308b\u9175\u7d20\u3067\u3042\u308a\u3001\u91d1\u5c5e\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b[1]\u3002 \u30db\u30b9\u30db\u30c8\u30ea\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08phosphotriesterase\u3001PTE\uff09\u3001\u6709\u6a5f\u30ea\u30f3\u52a0\u6c34\u5206\u89e3\u9175\u7d20\uff08organophosphorus hydrolase\uff09\u3001\u30d1\u30e9\u30c1\u30aa\u30f3\u52a0\u6c34\u5206\u89e3\u9175\u7d20\uff08parathion hydrolase\uff09\u3001\u30d1\u30e9\u30aa\u30ad\u30bd\u30ca\u30fc\u30bc\uff08paraoxonase\uff09\u3001\u30d1\u30e9\u30c1\u30aa\u30f3\u30a2\u30ea\u30fc\u30eb\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08parathion aryl esterase\uff09\u306a\u3069\u306e\u540d\u79f0\u3067\u3082\u77e5\u3089\u308c\u308b\u3002 \u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178 + \u6c34 \u21cc{displaystyle rightleftharpoons } \u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178 + \u30a2\u30ea\u30fc\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb \u5f93\u3063\u3066\u3001\u3053\u306e\u9175\u7d20\u306e\u57fa\u8cea\u306f\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178\u3068\u6c34\u306e2\u3064\u3001\u751f\u6210\u7269\u306f\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178\u3068\u30a2\u30ea\u30fc\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb\uff08\u30d5\u30a7\u30ce\u30fc\u30eb\uff09\u306e2\u3064\u3067\u3042\u308b\u3002 \u3053\u306e\u9175\u7d20\u3092\u30b3\u30fc\u30c9\u3059\u308b\u907a\u4f1d\u5b50\uff08opd\u3001organophosphate-degrading\uff09\u306f\u30b7\u30e5\u30fc\u30c9\u30e2\u30ca\u30b9Pseudomonas diminuta\u306b\u5185\u5728\u3059\u308b\u5de8\u5927\u30d7\u30e9\u30b9\u30df\u30c9\uff08pSC1\u300151","datePublished":"2020-04-06","dateModified":"2020-04-06","author":{"@type":"Person","@id":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/author\/lordneo#Person","name":"lordneo","url":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/author\/lordneo","image":{"@type":"ImageObject","@id":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","height":96,"width":96}},"publisher":{"@type":"Organization","name":"Enzyklop\u00e4die","logo":{"@type":"ImageObject","@id":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/11\/book.png","url":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/11\/book.png","width":600,"height":60}},"image":{"@type":"ImageObject","@id":"https:\/\/wikimedia.org\/api\/rest_v1\/media\/math\/render\/svg\/1c37b981df851b9e54e489e017b1481e37d418f3","url":"https:\/\/wikimedia.org\/api\/rest_v1\/media\/math\/render\/svg\/1c37b981df851b9e54e489e017b1481e37d418f3","height":"","width":""},"url":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/3550","about":["Wiki"],"wordCount":5229,"articleBody":"\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\uff08Aryldialkylphosphatase\u3001EC 3.1.8.1\uff09\u306f\u3001 \u6709\u6a5f\u30ea\u30f3\u7cfb\u6bba\u866b\u5264\u306e\u30ea\u30f3\u9178\u30c8\u30ea\u30a8\u30b9\u30c6\u30eb\u306e\u52a0\u6c34\u5206\u89e3\u3092\u89e6\u5a92\u3059\u308b\u9175\u7d20\u3067\u3042\u308a\u3001\u91d1\u5c5e\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b[1]\u3002 \u30db\u30b9\u30db\u30c8\u30ea\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08phosphotriesterase\u3001PTE\uff09\u3001\u6709\u6a5f\u30ea\u30f3\u52a0\u6c34\u5206\u89e3\u9175\u7d20\uff08organophosphorus hydrolase\uff09\u3001\u30d1\u30e9\u30c1\u30aa\u30f3\u52a0\u6c34\u5206\u89e3\u9175\u7d20\uff08parathion hydrolase\uff09\u3001\u30d1\u30e9\u30aa\u30ad\u30bd\u30ca\u30fc\u30bc\uff08paraoxonase\uff09\u3001\u30d1\u30e9\u30c1\u30aa\u30f3\u30a2\u30ea\u30fc\u30eb\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08parathion aryl esterase\uff09\u306a\u3069\u306e\u540d\u79f0\u3067\u3082\u77e5\u3089\u308c\u308b\u3002\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178 + \u6c34 \u21cc{displaystyle rightleftharpoons } \u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178 + \u30a2\u30ea\u30fc\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb\u5f93\u3063\u3066\u3001\u3053\u306e\u9175\u7d20\u306e\u57fa\u8cea\u306f\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178\u3068\u6c34\u306e2\u3064\u3001\u751f\u6210\u7269\u306f\u30b8\u30a2\u30eb\u30ad\u30eb\u30ea\u30f3\u9178\u3068\u30a2\u30ea\u30fc\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb\uff08\u30d5\u30a7\u30ce\u30fc\u30eb\uff09\u306e2\u3064\u3067\u3042\u308b\u3002\u3053\u306e\u9175\u7d20\u3092\u30b3\u30fc\u30c9\u3059\u308b\u907a\u4f1d\u5b50\uff08opd\u3001organophosphate-degrading\uff09\u306f\u30b7\u30e5\u30fc\u30c9\u30e2\u30ca\u30b9Pseudomonas diminuta\u306b\u5185\u5728\u3059\u308b\u5de8\u5927\u30d7\u30e9\u30b9\u30df\u30c9\uff08pSC1\u300151 kb\uff09\u306b\u5b58\u5728\u3057\u3066\u3044\u308b\u304c[2]\u3002\u907a\u4f1d\u5b50\u306f\u30d5\u30e9\u30dc\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u5c5eFlavobacterium sp. ATCC27551\u306a\u3069\u4ed6\u306e\u591a\u304f\u306e\u751f\u7269\u7a2e\u306b\u3082\u5b58\u5728\u3057\u3066\u3044\u308b\u304c\u3001\u305d\u3053\u3067\u3082\u67d3\u8272\u4f53\u5916\u30a8\u30ec\u30e1\u30f3\u30c8\uff08pSM55\u300143 kb\uff09\u306b\u30b3\u30fc\u30c9\u3055\u308c\u3066\u3044\u308b[2]\u3002 \u30ea\u30f3\u9178\u30a8\u30b9\u30c6\u30eb\u306f\u30ea\u30f3\u9178\u306e\u30a8\u30b9\u30c6\u30eb\u306e\u4e00\u822c\u540d\u79f0\u3067\u3042\u308a\u3001\u6709\u6a5f\u30ea\u30f3\u5316\u5408\u7269\u306e\u4e00\u3064\u3067\u3042\u308b\u3002\u6bba\u866b\u5264\u3001\u9664\u8349\u5264\u3001\u795e\u7d4c\u30ac\u30b9\u7b49\u306e\u6210\u5206\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u3002\u6bd2\u6027\u306e\u4f4e\u3044\u30ea\u30f3\u9178\u30a8\u30b9\u30c6\u30eb\u306f\u3001\u6eb6\u5a92\u3001\u53ef\u5851\u5264\u3001\u6975\u5727\u6dfb\u52a0\u5264\uff08\u82f1\u8a9e\u7248\uff09\u7b49\u306b\u7528\u3044\u3089\u308c\u308b\u3002 \u6709\u6a5f\u30ea\u30f3\u7cfb\u6bba\u866b\u5264\u306f\u4e16\u754c\u4e2d\u306e\u6bba\u866b\u5264\u4f7f\u7528\u91cf\u306e\u7d0438%\u3092\u5360\u3081\u308b[3]\u3002\u6709\u6a5f\u30ea\u30f3\uff08OP\uff09\u7cfb\u6bba\u866b\u5264\u3092\u5206\u89e3\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u7d30\u83cc\u306f\u3001\u4e16\u754c\u4e2d\u306e\u3055\u307e\u3056\u307e\u306a\u571f\u58cc\u8a66\u6599\u4e2d\u306b\u540c\u5b9a\u3055\u308c\u3066\u3044\u308b[3][4]\u3002\u6709\u6a5f\u30ea\u30f3\u7cfb\u6bba\u866b\u5264\u3092\u5206\u89e3\u3059\u308b\u7d30\u83cc\u7a2e\u306f\u30011973\u5e74\u306b\u30d5\u30a3\u30ea\u30d4\u30f3\u306e\u571f\u58cc\u8a66\u6599\u304b\u3089\u521d\u3081\u3066\u5358\u96e2\u3055\u308c\u305f\uff08Flavobacterium sp. ATCC27551\uff09[5]\u3002\u305d\u306e\u5f8c\u3001\u4ed6\u306e\u7a2e\u3082OP\u5206\u89e3\u80fd\u529b\u3092\u6301\u3064\u3053\u3068\u304c\u793a\u3055\u308c\u305f\u3002\u4f8b\u3068\u3057\u3066\u306f\u3001Pseudomonas diminuta\uff08\u30a2\u30e1\u30ea\u30ab\u5408\u8846\u56fd\u306e\u571f\u58cc\u8a66\u6599\u304b\u3089\u5358\u96e2\uff09\u3001Agrobacterium radiobacter\uff08\u30aa\u30fc\u30b9\u30c8\u30e9\u30ea\u30a2\u306e\u571f\u58cc\u8a66\u6599\u304b\u3089\u5358\u96e2\uff09\u3001Alteromonas haloplanktis\uff08\u30a2\u30e1\u30ea\u30ab\u5408\u8846\u56fd\u306e\u571f\u58cc\u8a66\u6599\u304b\u3089\u5358\u96e2\uff09\u3001Pseudomonas sp. WBC-3\uff08\u4e2d\u56fd\u306e\u571f\u58cc\u8a66\u6599\u304b\u3089\u5358\u96e2\uff09\u304c\u3042\u308b[3]\u3002\u5168\u30b2\u30ce\u30e0\u306e\u914d\u5217\u76f8\u540c\u6027\u691c\u7d22\u306b\u3088\u3063\u3066\u3001opd\u3068\u540c\u3058\u907a\u4f1d\u5b50\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u914d\u5217\u306f\u3001\u5927\u8178\u83ccEscherichia coli\uff08yhfV\uff09\u3084\u7d50\u6838\u83ccMycobacterium tuberculosis\u3001\u30a6\u30b7\u578b\u7d50\u6838\u83ccMycobacterium bovis\u306a\u3069\u306e\u75c5\u539f\u6027\u7d30\u83cc\u3001\u653e\u5c04\u7dda\u8010\u6027\u751f\u7269\u3068\u3057\u3066\u77e5\u3089\u308c\u308bDeinococcus radiodurans\u3001\u5acc\u6c17\u6027\u7d30\u83ccDesulfatibacillum alkenivorans\u3001\u597d\u71b1\u6027\u7d30\u83ccGeobacillus sp.\u3068Thermoanaerobacter sp. X514\u3001\u306a\u3069\u4ed6\u306e\u7d30\u83cc\u306b\u3082\u540c\u5b9a\u3055\u308c\u3066\u3044\u308b[3]\u3002\u6709\u6a5f\u30ea\u30f3\u3092\u52a0\u6c34\u5206\u89e3\u3059\u308b\u80fd\u529b\u306f\u7d30\u83cc\u306b\u9650\u5b9a\u3055\u308c\u306a\u3044\u3002\u3044\u304f\u3064\u304b\u306e\u83cc\u985e\u3068\u30b7\u30a2\u30ce\u30d0\u30af\u30c6\u30ea\u30a2\u3082OP\u3092\u52a0\u6c34\u5206\u89e3\u3059\u308b\u3053\u3068\u304c\u767a\u898b\u3055\u308c\u3066\u3044\u308b[3]\u3002\u307e\u305f\u3001\u76f8\u540c\u914d\u5217\u306fSulfolobus acidocaldarius\u306a\u3069\u4e00\u90e8\u306e\u53e4\u7d30\u83cc\u306b\u3082\u5b58\u5728\u3057\u3066\u3044\u308b[6]\u3002Flavobacterium sp. ATCC27551\u3068Pseudomonas diminuta\u306b\u5b58\u5728\u3059\u308b\u907a\u4f1d\u5b50\uff08opd\uff09\u306f\u9ad8\u5ea6\u306b\u4fdd\u5b58\u3055\u308c\u3066\u3044\u308b\u304c[4]\u3001\u907a\u4f1d\u5b50\u304c\u5b58\u5728\u3057\u3066\u3044\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u3001\u907a\u4f1d\u5b50\u304c\u5b58\u5728\u3059\u308b 5.1 kb\u306e\u4fdd\u5b58\u9818\u57df\u3092\u9664\u3044\u3066\u3001\u5927\u304d\u304f\u914d\u5217\u304c\u5927\u304d\u304f\u7570\u306a\u308b[2]\u3002 Flavobacterium\u306eopd\u907a\u4f1d\u5b50\u69cb\u6210\u306e\u6ce8\u610f\u6df1\u3044\u89b3\u5bdf\u304b\u3089\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u306e\u3088\u3046\u306b\u6a5f\u80fd\u3059\u308b\u53ef\u80fd\u6027\u306e\u3042\u308b\u69cb\u9020\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u3066\u3044\u308b\u3002\u3053\u306e\u3053\u3068\u306f\u3001\u4ed6\u306e\u7d30\u83cc\u7a2e\u9593\u3067\u306e\u907a\u4f1d\u5b50\u306e\u5e83\u7bc4\u306a\u5206\u5e03\u304cDNA\u306e\u6c34\u5e73\u4f1d\u64ad\u306b\u3088\u3063\u3066\u751f\u3058\u305f\u53ef\u80fd\u6027\u3092\u793a\u3057\u3066\u3044\u308b\u3002opd\u907a\u4f1d\u5b50\u306f\u3001Tn3\uff08\u82f1\u8a9e\u7248\uff09\u30d5\u30a1\u30df\u30ea\u30fc\u306e\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u306b\u7279\u5fb4\u7684\u306a\u8ee2\u79fb\u633f\u5165\u914d\u5217\u3068\u96a3\u63a5\u3057\u3066\u3044\u308b\u3002\u3055\u3089\u306b\u3001opd\u907a\u4f1d\u5b50\u306e\u4e0a\u6d41\u306b\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30dd\u30b6\u30fc\u30bc\uff08\u82f1\u8a9e\u7248\uff09\u69d8\u306e\u914d\u5217\uff08TnpA\u3068\u76f8\u540c\uff09\u3084\u30ec\u30be\u30eb\u30d0\u30fc\u30bc\u69d8\u306e\u914d\u5217\uff08TnpR\u3068\u76f8\u540c\uff09\u3082\u540c\u5b9a\u3055\u308c\u3066\u304a\u308a[4]\u3001\u3053\u308c\u3089\u306fTn3\u306a\u3069\u306e\u30af\u30e9\u30b9II\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u306b\u7279\u5fb4\u7684\u306a\u914d\u5217\u3067\u3042\u308b\u3002opd\u907a\u4f1d\u5b50\u306e\u4e0b\u6d41\u306b\u306f\u4ed6\u306eORF\u304c\u540c\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001OP\u306e\u5206\u89e3\u7523\u7269\u306e1\u3064\u3067\u3042\u308bp-\u30cb\u30c8\u30ed\u30d5\u30a7\u30ce\u30fc\u30eb\u3092\u5206\u89e3\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30b3\u30fc\u30c9\u3055\u308c\u3066\u3044\u308b\u3002\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u30db\u30b9\u30db\u30c8\u30ea\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08PTE\uff09\u306e\u5b58\u5728\u4e0b\u3067\u5287\u7684\u306a\u6d3b\u6027\u306e\u4e0a\u6607\u304c\u89b3\u5bdf\u3055\u308c\u308b\u305f\u3081\u3001PTE\u3068\u306e\u8907\u5408\u4f53\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b[4]\u3002Table of Contents\u30bf\u30f3\u30d1\u30af\u8cea[\u7de8\u96c6]\u69cb\u9020[\u7de8\u96c6]\u89e6\u5a92[\u7de8\u96c6]\u901f\u5ea6\u8ad6[\u7de8\u96c6]\u7d30\u80de\u5185\u5c40\u5728[\u7de8\u96c6]\u74b0\u5883\u306b\u5bfe\u3059\u308b\u91cd\u8981\u6027[\u7de8\u96c6]\u95a2\u9023\u6587\u732e[\u7de8\u96c6]\u30bf\u30f3\u30d1\u30af\u8cea[\u7de8\u96c6]\u69cb\u9020[\u7de8\u96c6]\u30db\u30b9\u30db\u30c8\u30ea\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\uff08PTE\uff09\u306f\u3001\u53cd\u5fdc\u306e\u89e6\u5a92\u3092\u884c\u30462\u3064\u306e\u4e9c\u925b\u30a4\u30aa\u30f3\u3092\u6301\u3064\u91d1\u5c5e\u9175\u7d20\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5c5e\u3059\u308b\u3002\u91d1\u5c5e\u539f\u5b50\u306f\u5171\u901a\u306e\u30ea\u30ac\u30f3\u30c9\u3092\u4ecb\u3057\u3066\u9023\u7d50\u3055\u308c\u3001\u5468\u56f2\u306b\u5bc6\u96c6\u3057\u305f\u30d2\u30b9\u30c1\u30b8\u30f3\u6b8b\u57fa\u306e\u30a4\u30df\u30c0\u30be\u30a4\u30eb\u57fa\u304c\u914d\u4f4d\u3057\u3066\u3044\u308b[7]\u3002\u3053\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u4e8c\u91cf\u4f53\u3092\u5f62\u6210\u3059\u308b[8]\u3002\u5168\u4f53\u69cb\u9020\u306f\u03b1\/\u03b2\u30d0\u30ec\u30eb\u30e2\u30c1\u30fc\u30d5\u304b\u3089\u69cb\u6210\u3055\u308c\u3001\u3053\u306e\u30e2\u30c1\u30fc\u30d5\u306f\u4ed6\u306e20\u7a2e\u985e\u306e\u89e6\u5a92\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3082\u5b58\u5728\u3057\u3066\u3044\u308b\u3002\u3053\u308c\u3089\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6d3b\u6027\u90e8\u4f4d\u306f\u03b2\u30d0\u30ec\u30eb\u306eC\u672b\u7aef\u90e8\u5206\u306b\u4f4d\u7f6e\u3057\u3001PTE\u3067\u3082\u540c\u69d8\u3067\u3042\u308b[7]\u3002 \u30d1\u30e9\u30aa\u30ad\u30bd\u30f3\u304b\u3089\u30b8\u30a8\u30c1\u30eb\u30ea\u30f3\u9178\u3068p-\u30cb\u30c8\u30ed\u30d5\u30a7\u30ce\u30fc\u30eb\u3078\u306e\u9175\u7d20\u89e6\u5a92\u306b\u3088\u308b\u52a0\u6c34\u5206\u89e3\u306e\u53cd\u5fdc\u30b9\u30ad\u30fc\u30e0\u3002\u89e6\u5a92[\u7de8\u96c6]\u6709\u6a5f\u30ea\u30f3\u306b\u5bfe\u3059\u308b\u89e6\u5a92\u306f\u3001\u57fa\u8cea\u306e\u30ea\u30f3\u4e2d\u5fc3\u306b\u95a2\u3057\u3066\u914d\u7f6e\u306e\u53cd\u8ee2\u3092\u4f34\u3046\u6c42\u6838\u7f6e\u63db\u53cd\u5fdc\uff08SN2\u6a5f\u69cb\uff09\u3092\u4ecb\u3057\u3066\u884c\u308f\u308c\u308b[7]\u3002\u6d3b\u6027\u90e8\u4f4d\u3067\u306f\u3001\u91d1\u5c5e\u30ab\u30c1\u30aa\u30f3\u304c\u57fa\u8cea\u306eP-O\u7d50\u5408\u3092\u3055\u3089\u306b\u5206\u6975\u5316\u3057\u3001\u6c42\u6838\u653b\u6483\u306b\u5bfe\u3059\u308b\u611f\u53d7\u6027\u3092\u9ad8\u3081\u308b\u3053\u3068\u3067\u89e6\u5a92\u3092\u88dc\u52a9\u3057\u3066\u3044\u308b\u3002\u3055\u3089\u306b\u3001\u5869\u57fa\u6027\u6b8b\u57fa\u304c\u6c34\u5206\u5b50\u304b\u3089\u30d7\u30ed\u30c8\u30f3\u3092\u5f15\u304d\u629c\u304d\u3001\u305d\u306e\u7d50\u679c\u5f62\u6210\u3055\u308c\u305f\u6c34\u9178\u5316\u7269\u30a4\u30aa\u30f3\uff08OH–\uff09\u304c2\u3064\u306e2\u4fa1\u30ab\u30c1\u30aa\u30f3\u3092\u6a4b\u6e21\u3057\u3057\u3001\u6c42\u6838\u5264\u3068\u3057\u3066\u6a5f\u80fd\u3059\u308b\u3002\u305d\u306e\u5f8c\u3001OH–\u306f\u57fa\u8cea\u306e\u30ea\u30f3\u4e2d\u5fc3\u3092\u653b\u6483\u3057\u3001\u7d9a\u3044\u3066\u30d7\u30ed\u30c8\u30f3\u79fb\u52d5\u304c\u8d77\u3053\u308b\u3002P-O\u7d50\u5408\u306f\u5207\u65ad\u3055\u308c\u3001\u53cd\u5fdc\u7523\u7269\u304c\u6d3b\u6027\u90e8\u4f4d\u304b\u3089\u653e\u51fa\u3055\u308c\u308b[9]\u3002\u30db\u30b9\u30db\u30c8\u30ea\u30a8\u30b9\u30c6\u30e9\u30fc\u30bc\u306e\u53cd\u5fdc\u56de\u8ee2\u6570\uff08kcat\uff09\u306f\u3001\u30d1\u30e9\u30aa\u30ad\u30bd\u30f3\u306e\u52a0\u6c34\u5206\u89e3\u306e\u5834\u5408\u307b\u307c 104 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opd\u907a\u4f1d\u5b50\u306e750\u5869\u57fa\u5bfe\u4e0b\u6d41\u306b\u30b3\u30fc\u30c9\u3055\u308c\u308b\u9175\u7d20\u306b\u3088\u3063\u3066\u3055\u3089\u306b\u5206\u89e3\u3055\u308c\u308b\u3002\u3053\u306e\u9175\u7d20\u306f 29 kDa\u306e\u52a0\u6c34\u5206\u89e3\u9175\u7d20\u3067\u3042\u308b\u3068\u63a8\u5b9a\u3055\u308c\u3066\u304a\u308a\u3001\u82b3\u9999\u65cf\u5316\u5408\u7269\u3092\u5206\u89e3\u3057\u3001PTE\u3068\u5354\u50cd\u7684\u306b\u6a5f\u80fd\u3059\u308b[4]\u3002\u3053\u306e\u9175\u7d20\u306fPseudomonas putida\u3001Pseudomonas azelaica\u3001Rhodococcus sp.\u3001P. 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a b \u201cExpression and subcellular localization of organophosphate hydrolase in acephate-degrading Pseudomonas sp. strain Ind01 and its use as a potential biocatalyst for elimination of organophosphate insecticides\u201d. 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PMID\u00a012732518. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC154515\/.\u00a0^ \u201cA Flavobacterium sp. that degrades diazinon and parathion\u201d. Canadian Journal of Microbiology 19 (7): 873\u20135. (July 1973). doi:10.1139\/m73-138. PMID\u00a04727806.\u00a0^ \u201cThe genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota\u201d. Journal of Bacteriology 187 (14): 4992\u20139. (July 2005). doi:10.1128\/JB.187.14.4992-4999.2005. PMC\u00a01169522. PMID\u00a015995215. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1169522\/.\u00a0^ a b c d \u201cThree-dimensional structure of phosphotriesterase: an enzyme capable of detoxifying organophosphate nerve agents\u201d. Biochemistry 33 (50): 15001\u20137. (December 1994). doi:10.1021\/bi00254a008. PMID\u00a07999757.\u00a0^ \u201cCrystal structure of methyl parathion hydrolase from Pseudomonas sp. WBC-3\u201d. Journal of Molecular Biology 353 (3): 655\u201363. (October 2005). doi:10.1016\/j.jmb.2005.08.057. PMID\u00a016181636.\u00a0^ a b \u201cMechanism for the hydrolysis of organophosphates by the bacterial phosphotriesterase\u201d. Biochemistry 43 (19): 5707\u201315. (May 2004). doi:10.1021\/bi0497805. PMID\u00a015134445.\u00a0^ \u201cPurification and characterization of three parathion hydrolases from gram-negative bacterial strains\u201d. Applied and Environmental Microbiology 55 (2): 289\u201393. (February 1989). PMC\u00a0184103. PMID\u00a02541658. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC184103\/.\u00a0^ a b Classen, John J.; Engler, Cady R.; Kenerley, Charles M.; Whittaker, A. Dale (April 2000). \u201cA logistic model of subsurface fungal growth with application to bioremediation\u201d. Journal of Environmental Science and Health, Part A 35 (4): 465\u2013488. doi:10.1080\/10934520009376982.\u00a0^ a b Caldwell, S. R.; Newcomb, J. R.; Schlecht, K. A.; Raushel, F. M. (1991-07-30). \u201cLimits of diffusion in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta\u201d. Biochemistry 30 (30): 7438\u20137444. doi:10.1021\/bi00244a010. ISSN\u00a00006-2960. PMID\u00a01649628. https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/1649628.\u00a0^ a b \u201cMicrobial degradation of organophosphorus compounds\u201d. FEMS Microbiology Reviews 30 (3): 428\u201371. (May 2006). doi:10.1111\/j.1574-6976.2006.00018.x. PMID\u00a016594965.\u00a0^ a b \u201cPurification and properties of the phosphotriesterase from Pseudomonas diminuta\u201d. The Journal of Biological Chemistry 264 (33): 19659\u201365. (November 1989). PMID\u00a02555328.\u00a0^ Lotti, Marcello (2002-12-27). \u201cPromotion of organophosphate induced delayed polyneuropathy by certain esterase inhibitors\u201d. Toxicology 181-182: 245\u2013248. doi:10.1016\/s0300-483x(02)00291-3. ISSN\u00a00300-483X. PMID\u00a012505319. https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/12505319.\u00a0^ Ragnarsdottir, K. V. (2000). \u201cEnvironmental fate and toxicology of organophosphate pesticides\u201d. Journal of the Geological Society 157 (4): 859\u2013876. doi:10.1144\/jgs.157.4.859.\u00a0\u95a2\u9023\u6587\u732e[\u7de8\u96c6]"},{"@context":"http:\/\/schema.org\/","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"item":{"@id":"https:\/\/wiki.edu.vn\/jp\/wiki2\/#breadcrumbitem","name":"Enzyklop\u00e4die"}},{"@type":"ListItem","position":2,"item":{"@id":"https:\/\/wiki.edu.vn\/jp\/wiki2\/archives\/3550#breadcrumbitem","name":"\u30a2\u30ea\u30fc\u30eb\u30b8\u30a2\u30eb\u30ad\u30eb\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc – Wikipedia"}}]}]