[{"@context":"http:\/\/schema.org\/","@type":"BlogPosting","@id":"https:\/\/wiki.edu.vn\/jp\/wiki22\/archives\/287861#BlogPosting","mainEntityOfPage":"https:\/\/wiki.edu.vn\/jp\/wiki22\/archives\/287861","headline":"\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3 – Wikipedia","name":"\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3 – Wikipedia","description":"\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3 (fluorescein) \u306f\u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u7528\u3044\u3089\u308c\u308b\u86cd\u5149\u8272\u7d20\u306e\u4e00\u7a2e\u3067\u3042\u308b\u3002\u4ed6\u306b\u3082\u8272\u7d20\u30ec\u30fc\u30b6\u30fc\u306e\u5a92\u4f53\u3001\u6cd5\u533b\u5b66\u3084\u8840\u6e05\u5b66\u306b\u304a\u3051\u308b\u8840\u75d5\u306e\u63a2\u7d22\u3001\u8272\u7d20\u8ffd\u8de1\uff08\u82f1\u8a9e\u7248\uff09\u7528\u9014\u306a\u3069\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u3002 \u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306f1871\u5e74\u3001\u30a2\u30c9\u30eb\u30d5\u30fb\u30d5\u30a9\u30f3\u30fb\u30d0\u30a4\u30e4\u30fc\u306b\u3088\u308a\u767a\u898b\u3055\u308c\u305f\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u5438\u53ce\u6975\u5927\u306f\u6ce2\u9577 494nm\uff08\u9752\u8272\u5149\uff09\u3001\u653e\u51fa\u3059\u308b\u86cd\u5149\u306e\u6975\u5927\u306f 521nm\uff08\u7dd1\u8272\u5149\u3001\u6c34\u4e2d\uff09\u3067\u3042\u308a\u3001\u52b1\u8d77\u306b\u306f\u30a2\u30eb\u30b4\u30f3\u30ec\u30fc\u30b6\u30fc\u306a\u3069\u304c\u5229\u7528\u3067\u304d\u308b\u3002\u307e\u305f\u7b49\u5438\u53ce\u70b9\uff08\u5168\u3066\u306epH\u3067\u7b49\u3057\u3044\u5438\u53ce\u3092\u793a\u3059\u70b9\uff09\u3092 460nm \u306b\u6301\u3064\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306b\u306f\u300c\u30ec\u30bd\u30eb\u30b7\u30ce\u30fc\u30eb\u30d5\u30bf\u30ec\u30a4\u30f3\u300d\u306a\u3069\u591a\u304f\u306e\u5225\u540d\u3084\u6163\u7528\u540d\u304c\u3042\u308b\u3002\u7740\u8272\u6599\u3068\u3057\u3066\u306f\u300cD\uff06C\u30a4\u30a8\u30ed\u30fc7\u300d\u304a\u3088\u3073\u300cD\uff06C\u30a4\u30a8\u30ed\u30fc8\u300d\uff08\u4e8c\u30ca\u30c8\u30ea\u30a6\u30e0\u5869\uff09\u306e\u540d\u3067\u77e5\u3089\u308c\u3066\u3044\u308b\u3002 Table of Contents \u5316\u5b66\u7684\u30fb\u7269\u7406\u7684\u7279\u6027[\u7de8\u96c6]\u5165\u6d74\u5264[\u7de8\u96c6]\u6cb3\u5ddd\u3067\u306e\u5229\u7528[\u7de8\u96c6]\u751f\u7269\u5b66[\u7de8\u96c6]\u773c\u79d1\u5b66[\u7de8\u96c6]\u95a2\u9023\u9805\u76ee[\u7de8\u96c6]\u5916\u90e8\u30ea\u30f3\u30af[\u7de8\u96c6] \u5316\u5b66\u7684\u30fb\u7269\u7406\u7684\u7279\u6027[\u7de8\u96c6] \u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u96fb\u96e2\u5e73\u8861\u306b\u304a\u3051\u308b pKa \u306f 6.4 \u3067\u3042\u308b\u3002\u5f93\u3063\u3066\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306f pH 5","datePublished":"2022-04-26","dateModified":"2022-04-26","author":{"@type":"Person","@id":"https:\/\/wiki.edu.vn\/jp\/wiki22\/archives\/author\/lordneo#Person","name":"lordneo","url":"https:\/\/wiki.edu.vn\/jp\/wiki22\/archives\/author\/lordneo","image":{"@type":"ImageObject","@id":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/c9645c498c9701c88b89b8537773dd7c?s=96&d=mm&r=g","height":96,"width":96}},"publisher":{"@type":"Organization","name":"Enzyklop\u00e4die","logo":{"@type":"ImageObject","@id":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/11\/book.png","url":"https:\/\/wiki.edu.vn\/wiki4\/wp-content\/uploads\/2023\/11\/book.png","width":600,"height":60}},"image":{"@type":"ImageObject","@id":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/7\/77\/Fluorescein_Uebersicht.svg\/500px-Fluorescein_Uebersicht.svg.png","url":"https:\/\/upload.wikimedia.org\/wikipedia\/commons\/thumb\/7\/77\/Fluorescein_Uebersicht.svg\/500px-Fluorescein_Uebersicht.svg.png","height":"188","width":"500"},"url":"https:\/\/wiki.edu.vn\/jp\/wiki22\/archives\/287861","about":["Wiki"],"wordCount":1016,"articleBody":"\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3 (fluorescein) \u306f\u9855\u5fae\u93e1\u89b3\u5bdf\u306b\u7528\u3044\u3089\u308c\u308b\u86cd\u5149\u8272\u7d20\u306e\u4e00\u7a2e\u3067\u3042\u308b\u3002\u4ed6\u306b\u3082\u8272\u7d20\u30ec\u30fc\u30b6\u30fc\u306e\u5a92\u4f53\u3001\u6cd5\u533b\u5b66\u3084\u8840\u6e05\u5b66\u306b\u304a\u3051\u308b\u8840\u75d5\u306e\u63a2\u7d22\u3001\u8272\u7d20\u8ffd\u8de1\uff08\u82f1\u8a9e\u7248\uff09\u7528\u9014\u306a\u3069\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u3002  \u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306f1871\u5e74\u3001\u30a2\u30c9\u30eb\u30d5\u30fb\u30d5\u30a9\u30f3\u30fb\u30d0\u30a4\u30e4\u30fc\u306b\u3088\u308a\u767a\u898b\u3055\u308c\u305f\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u5438\u53ce\u6975\u5927\u306f\u6ce2\u9577 494nm\uff08\u9752\u8272\u5149\uff09\u3001\u653e\u51fa\u3059\u308b\u86cd\u5149\u306e\u6975\u5927\u306f 521nm\uff08\u7dd1\u8272\u5149\u3001\u6c34\u4e2d\uff09\u3067\u3042\u308a\u3001\u52b1\u8d77\u306b\u306f\u30a2\u30eb\u30b4\u30f3\u30ec\u30fc\u30b6\u30fc\u306a\u3069\u304c\u5229\u7528\u3067\u304d\u308b\u3002\u307e\u305f\u7b49\u5438\u53ce\u70b9\uff08\u5168\u3066\u306epH\u3067\u7b49\u3057\u3044\u5438\u53ce\u3092\u793a\u3059\u70b9\uff09\u3092 460nm \u306b\u6301\u3064\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306b\u306f\u300c\u30ec\u30bd\u30eb\u30b7\u30ce\u30fc\u30eb\u30d5\u30bf\u30ec\u30a4\u30f3\u300d\u306a\u3069\u591a\u304f\u306e\u5225\u540d\u3084\u6163\u7528\u540d\u304c\u3042\u308b\u3002\u7740\u8272\u6599\u3068\u3057\u3066\u306f\u300cD\uff06C\u30a4\u30a8\u30ed\u30fc7\u300d\u304a\u3088\u3073\u300cD\uff06C\u30a4\u30a8\u30ed\u30fc8\u300d\uff08\u4e8c\u30ca\u30c8\u30ea\u30a6\u30e0\u5869\uff09\u306e\u540d\u3067\u77e5\u3089\u308c\u3066\u3044\u308b\u3002Table of Contents\u5316\u5b66\u7684\u30fb\u7269\u7406\u7684\u7279\u6027[\u7de8\u96c6]\u5165\u6d74\u5264[\u7de8\u96c6]\u6cb3\u5ddd\u3067\u306e\u5229\u7528[\u7de8\u96c6]\u751f\u7269\u5b66[\u7de8\u96c6]\u773c\u79d1\u5b66[\u7de8\u96c6]\u95a2\u9023\u9805\u76ee[\u7de8\u96c6]\u5916\u90e8\u30ea\u30f3\u30af[\u7de8\u96c6]\u5316\u5b66\u7684\u30fb\u7269\u7406\u7684\u7279\u6027[\u7de8\u96c6]\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u96fb\u96e2\u5e73\u8861\u306b\u304a\u3051\u308b pKa \u306f 6.4 \u3067\u3042\u308b\u3002\u5f93\u3063\u3066\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306f pH 5 \u301c 9 \u306e\u7bc4\u56f2\u3067 pH \u4f9d\u5b58\u6027\u306e\u5438\u53ce\u3068\u86cd\u5149\u653e\u51fa\u3092\u793a\u3059\u3002\u307e\u305f\u3001\u30d7\u30ed\u30c8\u30f3\u5316\/\u8131\u30d7\u30ed\u30c8\u30f3\u5316\u3055\u308c\u305f\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u86cd\u5149\u5bff\u547d\u306f\u304a\u3088\u305d 3 \u301c 4 ns \u3067\u3042\u308a\u3001\u3053\u308c\u3092\u5229\u7528\u3057\u3066 pH \u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u308b\u3002\u3053\u306e\u5bff\u547d\u306f\u6642\u9593\u76f8\u95a2\u5358\u4e00\u5149\u5b50\u8a08\u6570\u6cd5 (time-correlated single photon counting; TCSPC) \u3084\u86cd\u5149\u5bff\u547d\u6e2c\u5b9a\u88c5\u7f6e\u306b\u3088\u3063\u3066\u77e5\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002  \u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306b\u306f\u3001\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u30a4\u30bd\u30c1\u30aa\u30b7\u30a2\u30cd\u30fc\u30c8 (fluorescein isothiocyanate; FITC) \u306b\u4ee3\u8868\u3055\u308c\u308b\u591a\u304f\u306e\u8a98\u5c0e\u4f53\u304c\u5b58\u5728\u3059\u308b\u3002FITC\u306f\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u6c34\u7d20\u539f\u5b50\u306e\u4e00\u3064\u3092\u30a4\u30bd\u30c1\u30aa\u30b7\u30a2\u30cd\u30fc\u30c8\u57fa\u3067\u7f6e\u63db\u3057\u305f\u5316\u5408\u7269\uff08\u53f3\u56f3\u53c2\u7167\uff09\u3067\u3042\u308a\u3001\u3053\u306e\u90e8\u4f4d\u3067\u30c1\u30aa\u5c3f\u7d20\u7d50\u5408\u3092\u5f62\u6210\u3057\u3001\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7b2c\u4e00\u7d1a\u30a2\u30df\u30f3\u3068\u7d50\u5408\u3059\u308b\u3002\u307e\u305f\u3001\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u9aa8\u683c\u306b\u30b9\u30af\u30b7\u30f3\u30a4\u30df\u30b8\u30eb\u30a8\u30b9\u30c6\u30eb\u3092\u4ed8\u52a0\u3057\u305fN-\u30d2\u30c9\u30ed\u30ad\u30b7\u30b9\u30af\u30b7\u30f3\u30a4\u30df\u30c9 (N-hydroxysuccinimide; NHS) \u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u3082\u30a2\u30df\u30f3\u306b\u5bfe\u3059\u308b\u7d50\u5408\u6027\u3092\u6301\u3063\u3066\u304a\u308a\u3001\u826f\u304f\u7528\u3044\u3089\u308c\u308b\u5316\u5408\u7269\u306e\u4e00\u3064\u3067\u3042\u308b\u3002\u305d\u306e\u4ed6\u306e\u8a98\u5c0e\u4f53\u3068\u3057\u3066\u306f\u3001\u30aa\u30ec\u30b4\u30f3\u30b0\u30ea\u30fc\u30f3\u3001\u30c8\u30fc\u30ad\u30e7\u30fc\u30b0\u30ea\u30fc\u30f3[1]\u3001SNAFL\u3001\u30ab\u30eb\u30dc\u30ad\u30b7\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u3001\u30ab\u30eb\u30dc\u30ad\u30b7\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u30b8\u30a2\u30bb\u30c6\u30fc\u30c8 (carboxy fluorescein diacetate; CFDA) \u306a\u3069\u304c\u3042\u308b\u3002\u3053\u308c\u3089\u306e\u8a98\u5c0e\u4f53\u306fAlexa (Molecular Probes) \u3084 DyLight\uff08\u30d4\u30a2\u30b9\u793e\uff09\u3068\u3044\u3063\u305f\u65b0\u53c2\u306e\u8272\u7d20\u3068\u5171\u306b\u3001\u3088\u308a\u9ad8\u3044\u5149\u5b89\u5b9a\u6027\u3001\u591a\u5f69\u306a\u30b9\u30da\u30af\u30c8\u30eb\u7279\u6027\u30fb\u7d50\u5408\u7279\u6027\u304c\u8981\u6c42\u3055\u308c\u308b\u5316\u5b66\u30fb\u751f\u7269\u5b66\u7528\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u4ed5\u7acb\u3066\u3089\u308c\u3001\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u5408\u6210\u306f\u7121\u6c34\u30d5\u30bf\u30eb\u9178\u3068\u30ec\u30bd\u30eb\u30b7\u30ce\u30fc\u30eb\u3092\u53cd\u5fdc\u7269\u3068\u3057\u3001\u5869\u5316\u4e9c\u925b\u306e\u5b58\u5728\u4e0b\u3067\u30d5\u30ea\u30fc\u30c7\u30eb\u30fb\u30af\u30e9\u30d5\u30c4\u53cd\u5fdc\u3092\u7d4c\u3066\u884c\u308f\u308c\u308b\u3002\u53cd\u5fdc\u89e6\u5a92\u3068\u3057\u3066\u306f\u3001\u5869\u5316\u4e9c\u925b\u306e\u4ed6\u306b\u30b9\u30eb\u30db\u30f3\u9178\u3082\u7528\u3044\u3089\u308c\u308b\u3002        \u5165\u6d74\u5264[\u7de8\u96c6]\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306f\u5165\u6d74\u5264\u306b\u6dfb\u52a0\u3059\u308b\u7740\u8272\u6599\u3068\u3057\u3066\u4f7f\u308f\u308c\u308b\u3002\u65e5\u672c\u306b\u304a\u3051\u308b\u3053\u306e\u3088\u3046\u306a\u4f7f\u7528\u4f8b\u306b\u3064\u3044\u3066\u306f\u3001\u85ac\u4e8b\u6cd5\u306e\u898f\u5b9a\u306b\u57fa\u3065\u3044\u305f\u3001\u533b\u85ac\u54c1\u7b49\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u30bf\u30fc\u30eb\u8272\u7d20\u3092\u5b9a\u3081\u308b\u7701\u4ee4\u306b\u3088\u3063\u3066\u9ec4\u8272201\u53f7\u3068\u3057\u3066\u6307\u5b9a\u3055\u308c\u3066\u3044\u308b\u3002\u6cb3\u5ddd\u3067\u306e\u5229\u7528[\u7de8\u96c6]\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u6700\u3082\u5927\u3005\u7684\u306a\u5229\u7528\u306f\u8056\u30d1\u30c8\u30ea\u30c3\u30af\u306e\u795d\u65e5\u306b\u898b\u3089\u308c\u308b\u3002\u30b7\u30ab\u30b4\u5ddd\u306b\u3053\u306e\u8272\u7d20\u3092\u6295\u5165\u3057\u3066\u7dd1\u8272\u306b\u67d3\u3081\u308b\u306e\u3067\u3042\u308b\u30021962\u5e74\u306b\u306f 45kg \u306e\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u304c\u6295\u5165\u3055\u308c\u3066\u3044\u308b\u304c\u30012005\u5e74\u3067\u306f 18kg 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Urano Y, Kamiya M, Kanda K, Ueno T, Hirose K, Nagano T (2005). \u201cEvolution of Fluorescein as a Platform for Finely Tunable Fluorescence Probes\u201d. 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